PTP1B 蛋白, Human (His)

PTP1B is an important tyrosine protein phosphatase that regulates the endoplasmic reticulum unfolded protein response by dephosphorylating EIF2AK3/PERK and inhibiting its kinase activity. It affects the signaling cascade induced by CKII and p60c-src, and may regulate the EFNA5-EPHA3 pathway, affecting cell reorganization. PTP1B Protein, Human (His) is the recombinant human-derived PTP1B protein, expressed by E. coli , with N-His labeled tag. The total length of PTP1B Protein, Human (His) is 320 a.a., with molecular weight of ~38.1 kDa.

PTP1B 是一种酪氨酸蛋白磷酸酶，是内质网未折叠蛋白反应的关键调节因子。它通过介导 EIF2AK3/PERK 的去磷酸化发挥作用，从而使 EIF2AK3/PERK 的蛋白激酶活性失活。PTP1B 在 CKII 和 p60c-src 诱导的信号转导级联中发挥潜在作用。此外，它可能对 EFNA5-EPHA3 信号通路产生调节影响，参与细胞重组和细胞间排斥。此外，PTP1B 被认为可以通过其去磷酸化 MET 的能力来调节肝细胞生长因子受体信号通路。这些多方面的功能强调了 PTP1B 在协调不同细胞过程和信号通路方面的多功能性。

Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate and the specific activity is > 15 nmoles/min/μg.

Human

E. coli

N-His

P18031 (E2-N321)

5770  [NCBI]

Approximately 38.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 10 mM HEPES, 150 mM NaCl, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.