PTP4A2 蛋白, Human (His)

The PTP4A2 protein is an influential tyrosine phosphatase that stimulates G1 to S phase progression and contributes to cell cycle regulation. Notably, it promotes tumorigenesis, revealing its involvement in cancer development. PTP4A2 Protein, Human (His) is the recombinant human-derived PTP4A2 protein, expressed by E. coli , with C-6*His labeled tag. The total length of PTP4A2 Protein, Human (His) is 167 a.a., with molecular weight of ~20 kDa.

PTP4A2 蛋白是一种有效的蛋白酪氨酸磷酸酶，在刺激有丝分裂期间从 G1 进入 S 期方面发挥着关键作用，有助于细胞周期调节。有趣的是，PTP4A2 作为肿瘤发生的启动子出现，表明它参与了与癌症发展相关的病理过程。此外，PTP4A2 通过破坏 RABGGTA 和 RABGGTB 之间的关联，对香叶基香叶基转移酶 II 型活性表现出抑制作用，表明其对细胞内信号通路的调节影响。PTP4A2 的多方面功能强调了其在与细胞周期动力学和肿瘤进展的复杂机制相关的细胞过程中的重要性。

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). which can be measured by absorbance at 410 nm. The specific activity is 158.928 pmol/min/µg, as measured under the described conditions.

Human

E. coli

C-6*His

Q12974 (M1-Q167)

8073  [NCBI]

MNRPAPVEISYENMRFLITHNPTNATLNKFTEELKKYGVTTLVRVCDATYDKAPVEKEGIHVLDWPFDDGAPPPNQIVDDWLNLLKTKFREEPGCCVAVHCVAGLGRAPVLVALALIECGMKYEDAVQFIRQKRRGAFNSKQLLYLEKYRPKMRLRFRDTNGHCCVQ

Approximately 20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.