PTPMT1 蛋白, Human (His)

The PTPMT1 protein is a lipid phosphatase that critically regulates cellular lipid metabolism by dephosphorylating phosphatidylglycerolphosphate (PGP) to form phosphatidylglycerol (PG). This step is critical for the biosynthesis of cardiolipin, a key mitochondrial phospholipid that regulates membrane integrity and mitochondrial activity. PTPMT1 Protein, Human (His) is the recombinant human-derived PTPMT1 protein, expressed by E. coli , with N-His labeled tag.

PTPMT1 蛋白作为脂质磷酸酶发挥作用，在细胞脂质代谢中发挥关键作用。它催化磷脂酰甘油磷酸 (PGP) 去磷酸化为磷脂酰甘油 (PG)，这是心磷脂生物合成途径中的关键步骤，心磷脂是调节膜完整性和线粒体活性的重要线粒体磷脂。除了与脂质相关的功能外，PTPMT1 对磷蛋白底物还表现出磷酸酶活性，特别是参与线粒体蛋白的去磷酸化。这种活性对于 ATP 的产生至关重要，并在胰腺 β 细胞胰岛素分泌的调节中发挥作用。此外，PTPMT1 可能通过调节线粒体膜完整性来防止内在细胞凋亡。

Measured by its ability to cleave pNPP. The specific activity is 666.88 pmoles/min/μg.

Human

E. coli

N-His

Q8WUK0-1 (K28-T201)

114971  [NCBI]

KVPGRAHRDWYHRIDPTVLLGALPLRSLTRQLVQDENVRGVITMNEEYETRFLCNSSQEWKRLGVEQLRLSTVDMTGIPTLDNLQKGVQFALKYQSLGQCVYVHCKAGRSRSATMVAAYLIQVHKWSPEEAVRAIAKIRSYIHIRPGQLDVLKEFHKQITARATKDGTFVISKT

Approximately 22 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.