RAB11B 蛋白, Human (HEK293, His)

RAB11B protein is an important small GTPase in the Rab family, coordinating intracellular membrane transport by regulating vesicle processes. It cycles between GDP and GTP forms, recruiting effectors to control activities such as vesicle formation and movement. RAB11B Protein, Human (HEK293, His) is the recombinant human-derived RAB11B protein, expressed by HEK293 , with N-His labeled tag. The total length of RAB11B Protein, Human (HEK293, His) is 214 a.a., with molecular weight of ~25-30 kDa.

小 GTP 酶 RAB11B 是 Rab 家族的成员，在细胞内膜运输中发挥着关键作用，协调从运输囊泡的形成到与膜融合的过程。这种 GTP 酶经历非活性 GDP 结合形式和活性 GTP 结合形式之间的循环，招募直接控制囊泡活动（例如形成、运动、束缚和融合）的不同下游效应器。具体来说，RAB11B 参与内吞再循环，调节跨膜蛋白的顶端再循环，包括囊性纤维化跨膜电导调节因子 (CFTR)、上皮钠通道 (ENaC)、钾电压门控通道和电压依赖性 L 型钙渠道。此外，它还参与组成型和调节性分泌的控制，例如胰岛素颗粒胞吐作用。RAB11B 对于黑素体运输和黑素细胞释放至关重要，并参与调节 V-ATP 酶细胞内运输以应对细胞外酸中毒。此外，它还促进 Rabin8/RAB3IP 前纤毛囊泡运输至母体中心粒，形成包括 Rab11、ASAP1、Rabin8/RAB3IP、RAB11FIP3 和 ARF4 的纤毛靶向复合物，从而调节纤毛发生的启动。相反，在 LPAR1 受体信号通路激活下，与磷酸化 WDR44 的相互作用会抑制 Rab11-RAB3IP-RAB11FIP3 复合物形成并抑制纤毛生长。

Measured by its ability to catalyze the substrate GTP. The specific activity is 0.27 nmol/min/mg.

Human

HEK293

N-6*His

Q15907-1 (G2-C215)

9230  [NCBI]

GTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCC

Approximately 25-30 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.