RAB1B 蛋白, Human (HEK293, Fc)

The RAB1B protein is an important small GTPase that regulates intracellular membrane trafficking by coordinating vesicular processes through cycling between GDP and GTP forms. It recruits downstream effectors to achieve vesicle functions, including formation, movement, and fusion. RAB1B Protein, Human (HEK293, Fc) is the recombinant human-derived RAB1B protein, expressed by HEK293 , with N-hFc labeled tag. The total length of RAB1B Protein, Human (HEK293, Fc) is 199 a.a., with molecular weight of ~58 kDa.

小 GTP 酶 Rab1B 是细胞内膜运输的关键调节因子，协调从运输囊泡形成到与膜融合的各个阶段。Rab1B 通过非活性 GDP 结合形式和活性 GTP 结合形式之间的循环进行操作，在招募负责囊泡过程（包括形成、运动、束缚和融合）的下游效应器方面发挥着关键作用。值得注意的是，Rab1B 有助于自噬泡发育的早期阶段，特别是在内质网的特殊区域。此外，它还参与调节内质网和连续高尔基体区室之间的囊泡运输，影响高尔基体的致密形态并促进脂质磷酸酶 MTMR6 募集到内质网-高尔基体中间区室。

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 3.355 ng/mL, corresponding to a specific activity is 2.98×10⁵ units/mg.

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  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 3.355 ng/mL, corresponding to a specific activity is 2.98×10⁵ units/mg.

Human

HEK293

N-hFc

Q9H0U4 (M1-G199)

81876  [NCBI]

MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGG

Approximately 58 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.