REG4 蛋白, Rat (HEK293, His)

The REG4 protein is a calcium-dependent lectin that functions as a pattern recognition receptor (PRR) in the innate immune system. REG4 has specific affinity for α-mannan on Candida albicans hyphae and triggers phosphorylation of the FCER1G immunoreceptor tyrosine activation motif (ITAM), thereby activating SYK, CARD9 and NF-kappa-B. REG4 Protein, Rat (HEK293, His) is the recombinant rat-derived REG4 protein, expressed by HEK293 , with C-His labeled tag.

Dectin-2/CLEC6A 是一种钙依赖性凝集素，在先天免疫系统中充当模式识别受体 (PRR)，对白色念珠菌菌丝上存在的 α-甘露聚糖具有特定的亲和力。结合后，该受体复合物会触发 FCER1G 免疫受体酪氨酸激活基序 (ITAM) 的磷酸化，从而激活 SYK、CARD9 和 NF-kappa-B。该激活序列导致抗原呈递细胞成熟，并针对效应 T 辅助细胞 1 和 T 辅助细胞 17 细胞亚型形成抗原特异性 T 细胞启动。此外，Dectin-2/CLEC6A 以甘露糖依赖性方式识别来自屋尘螨和真菌的过敏原，促进半胱氨酰白三烯的产生。它还通过识别曼氏血吸虫卵中的可溶性元素，在改变适应性免疫反应中发挥作用。它与 FCER1G 结合，与 CLEC4D 形成异二聚体，产生抗真菌感染的 PRR。

Measured by the ability of the immobilized protein to support the adhesion of HCT-116 human colorectal carcinoma cells under low serum conditions. The ED₅₀ for this effect is 2.633 µg/mL, corresponding to a specific activity is 379.79 units/mg.

Rat

HEK293

C-6*His

Q68AX7 (D23-P157)

445583  [NCBI]

DILRPSCASGWFNYRSHCYGYFRKLRNWSHAELECQSYGNGSHLASVLNPKEASVISKYITGYQRSLPVWIGLHDPQKNASWQWIDGSTNQYRPWSPRTKSEARHCTEMNPKDKFLTWNKNGCTKRQHFLCKYRP

Approximately 20-31 kDa due to the glycosylation.

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  Measured by the ability of the immobilized protein to support the adhesion of HCT-116 human colorectal carcinoma cells under low serum conditions. The ED₅₀ for this effect is 2.633 µg/mL, corresponding to a specific activity is 379.79 units/mg.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.