1. 重组蛋白
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. Protease-activated Receptor
  5. Renin 蛋白, Human (HEK293, His)

Renin 蛋白, Human (HEK293, His)

目录号: HY-P71052
COA 产品使用指南

肾素是一种特殊的肽链内切酶,能够独特地将血管紧张素原转化为血管紧张素 I,启动级联反应,从而升高血压并增强肾钠潴留。肾素启动血管紧张素通路的精确性强调了其在调节血压和电解质平衡中的关键作用。Renin 蛋白, Human (HEK293, His) 是重组的 肾素蛋白,由 HEK293 表达,带有 C-10*His 标签。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥380 In-stock
10 μg ¥650 In-stock
50 μg ¥1800 In-stock
100 μg ¥3060 In-stock
250 μg ¥5800 In-stock
500 μg ¥9800 In-stock
1 mg ¥14000 In-stock
> 1 mg   询价  

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Renin Protein, Human (HEK293, His) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Renin, a specialized endopeptidase, uniquely converts angiotensinogen to angiotensin I, initiating a cascade that elevates blood pressure and enhances kidney sodium retention. Renin's precision in initiating the angiotensin pathway underscores its pivotal role in regulating blood pressure and electrolyte balance. Renin Protein, Human (HEK293, His) is the recombinant human-derived Renin protein, expressed by HEK293 , with C-10*His labeled tag.

研究背景

肾素是一种特殊的肽链内切酶,在血浆内将血管紧张素原转化为血管紧张素 I 方面发挥着独特的功能。这种酶活性引发一系列反应,导致血压升高和肾脏钠潴留增加。肾素在启动血管紧张素通路方面的精确性强调了其在调节体内血压和电解质平衡方面的关键作用。

生物活性

Measured by its ability to cleave substrateArg-Glu(EDANS)-Ile-His-Pro-Phe-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg(HY-P5123A) that Incubate at 37 °C for 1 hour. The specific activity is ≥29.21 pmol/min/μg.

种属

Human

表达系统

HEK293

标签

C-10*His

蛋白编号

P00797-1 (L24-R406)

基因 ID
蛋白结构
N-term
Renin (L24-R406)
Accession # P00797-1
10*His
C-term
同用名
Renin; Angiotensinogenase; REN
氨基酸序列

LPTDTTTFKRIFLKRMPSIRESLKERGVDMARLGPEWSQPMKRLTLGNTTSSVILTNYMDTQYYGEIGIGTPPQTFKVVFDTGSSNVWVPSSKCSRLYTACVYHKLFDASDSSSYKHNGTELTLRYSTGTVSGFLSQDIITVGGITVTQMFGEVTEMPALPFMLAEFDGVVGMGFIEQAIGRVTPIFDNIISQGVLKEDVFSFYYNRDSENSQSLGGQIVLGGSDPQHYEGNFHYINLIKTGVWQIQMKGVSVGSSTLLCEDGCLALVDTGASYISGSTSSIEKLMEALGAKKRLFDYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFQESYSSKKLCTLAIHAMDIPPPTGPTWALGATFIRKFYTEFDRRNNRIGFALAR

分子量

43-50 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 8% Sucrose, 5% Mannitol, 0.05% Tween80, 100 mM NaCl, pH 7.4 or PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Renin Protein, Human (HEK293, His)
目录号:
HY-P71052
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