Serpin A4 蛋白, Human (HEK293, His)

Serpin A4 Protein inhibits tissue kallikrein's amidolytic and kininogenase activities by forming a heat- and SDS-stable equimolar complex with the enzyme. Tissue kallikrein concurrently cleaves the reactive site of Serpin A4, producing a small C-terminal fragment. The protein, existing as a monomer and occasionally forming homodimers, demonstrates its regulatory role in modulating tissue kallikrein-mediated enzymatic activities. Serpin A4 Protein, Human (HEK293, His) is the recombinant human-derived Serpin A4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Serpin A4 Protein, Human (HEK293, His) is 407 a.a., with molecular weight of 60-80 kDa.

Serpin A4 蛋白作为抑制剂，专门针对组织激肽释放酶的酰胺分解和激肽原酶活性。这种抑制机制涉及抑制剂和酶之间形成等摩尔复合物，该复合物对热和 SDS 稳定。同时，组织激肽释放酶裂解丝氨酸蛋白酶抑制剂 A4 的反应位点，产生一个小的 C 末端片段。该蛋白质以单体形式存在，在某些情况下形成同二聚体，显示出其在调节组织激肽释放酶介导的酶活性中的调节作用。

Measured by its ability to inhibit KLK1（ HY-P73262）cleavage of a colorimetric peptide substrate, Pro-Phe-Arg-7-amido-4-methylcoumarin（HY-137784）that incubate at 37℃ for 60 minutes. The IC₅₀ value is 2.59 nM.

Human

HEK293

C-6*His

P29622 (Q21-P427)

5267  [NCBI]

QLHVEHDGESCSNSSHQQILETGEGSPSLKIAPANADFAFRFYYLIASETPGKNIFFSPLSISAAYAMLSLGACSHSRSQILEGLGFNLTELSESDVHRGFQHLLHTLNLPGHGLETRVGSALFLSHNLKFLAKFLNDTMAVYEAKLFHTNFYDTVGTIQLINDHVKKETRGKIVDLVSELKKDVLMVLVNYIYFKALWEKPFISSRTTPKDFYVDENTTVRVPMMLQDQEHHWYLHDRYLPCSVLRMDYKGDATVFFILPNQGKMREIEEVLTPEMLMRWNNLLRKRNFYKKLELHLPKFSISGSYVLDQILPRLGFTDLFSKWADLSGITKQQKLEASKSFHKATLDVDEAGTEAAAATSFAIKFFSAQTNRHILRFNRPFLVVIFSTSTQSVLFLGKVVDPTKP

60-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 10 mM NaCl, pH 8.0 or 20 mM PB, 6% Trehalose, 4% Mannitol, 50 mM NaCl, 0.05% Tween 80, pH 6.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.