Serpin E1 蛋白, Rat (HEK293, His)

Serpin E1 Protein, Rat (HEK293, His) is one important member of the serine proteinase inhibitor E superfamily that plays a crucial role in the fibrinolytic system. The Serpin E1 protein is a single-chain glycoprotein composed of 379 amino acids. The active site of the protein is Arg346-Met347. SERPINE1 encodes plasminogen activator inhibitor type 1 (PAI-1), which serves as the primary inhibitor of urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA). The Serpin E1 protein can quickly inhibit the formation of plasmin. Serpin E1 can be transcribed through several signaling cascades, including pro-fibrogenic and pro-inflammatory pathways. Serpin E1 is a serine proteinase inhibitor, serves as an important regulator of extracellular matrix remodeling^[1][2][3]. Serpin E1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin E1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of Serpin E1 Protein, Rat (HEK293, His) is 379 a.a., with molecular weight of 48-60 kDa.

SERPINE1蛋白是由379个氨基酸组成的单链糖蛋白，主要由血小板、巨核细胞、肝细胞、脂肪细胞、平滑肌细胞和血管内皮细胞合成和分泌。该蛋白的活性位点是Arg346-Met347。激活的 SERPINE1 在 37°C 下的半衰期为 1-2 小时，自发转化为潜在形式。在纤溶系统中，SERPINE1 作为纤维蛋白原激活剂的抑制剂发挥生理作用。SerpinE1 在 Hutchinson-Gilford 早衰综合征中驱动细胞自主致病信号传导^[1]。
SERPINE1 被确定为胶质母细胞瘤细胞分散的调节因子，SERPINE1 的下调限制了胶质瘤细胞的增殖和侵袭^[3]。

Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC₅₀ value is < 80 nM.

Rat

HEK293

C-His;C-10*His

NP_036752.1 (S24-P402)

24617  [NCBI]

SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTTAGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQRDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVPMMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAITNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFSSTQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPTEMVLDRSFLFVVRHNPTETILFMGQLMEP

48-60 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.