Serpin F1 蛋白, Rat (HEK293, His)

Serpin F1 Protein, part of the secreted frizzled-related protein (sFRP) family, modulates Wnt signaling pathways. As a member, Serpin F1 likely interacts with Wnt ligands and frizzled receptors, regulating crucial cellular processes. The unique structural characteristics of Serpin F1 within the sFRP family, marked by the absence of conserved residue(s) for feature annotation propagation, underscore its distinctive role in Wnt signaling modulation. Despite lacking certain conserved elements, Serpin F1's specific attributes may contribute to its distinct influence on cellular activities, such as proliferation, differentiation, or tissue development. Serpin F1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin F1 protein, expressed by HEK293, with C-His labeled tag. The total length of Serpin F1 Protein, Rat (HEK293, His) is 399 a.a., with molecular weight of ~45.9 kDa.

SFRP2 蛋白是分泌型卷曲相关蛋白 (sFRP) 家族的成员，表明其参与调节 Wnt 信号通路。作为该家族的一部分，SFRP2 可能与 Wnt 配体和卷曲受体相互作用，对关键的细胞过程发挥调节作用。特征注释传播所需的保守残基的缺失强调了 sFRP 家族中 SFRP2 的独特结构特征。尽管缺乏某些保守元件，SFRP2 的特定属性可能有助于其在调节 Wnt 信号传导中发挥独特的作用，从而可能影响增殖、分化或组织发育等细胞活动。

Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED₅₀ this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×10³ units/mg.

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  Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED₅₀ this effect is 0.178 μg/mL, corresponding to a specific activity is 5.62×10³ units/mg.

Rat

HEK293

C-His

Q80ZA3/NP_808788.1 (Q20-T418)

287526  [NCBI]

QNVPDSSQDSPAPDSTGEPVVEEDDPFFKAPVNKLAAAVSNFGYDLYRLRSGAVSTGNILLSPLSVATALSALSLGAEQRTESVIHRALYYDLINNPDIHSTYKELLASVTAPEKNFKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRIDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWATKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFVHDIDRELKTIQAVLTVPKLKLSYEGDVTNSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGTSSNPDLQPVRLTFPLDYHLNRPFIFVLRDTDTGALLFIGRILDPSST

Approximately 45.9 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.