Siglec-11 蛋白, Human (E84A, K145Q, CHO, hFc)

Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc) is the recombinant human-derived Siglec-11 protein, expressed by CHO , with C-hFc labeled tag. The total length of Siglec-11 Protein, Human (E84A, K145Q, CHO, hFc) is 527 a.a., with molecular weight of 110-135 kDa.

Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-11 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human Siglec-15 Protein. The ED₅₀ for this effect is 0.975 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-11 Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human Siglec-15 Protein. The ED₅₀ for this effect is 0.975 μg/mL.

Human

CHO

C-hFc

AAK72907 (N17-H543, E84A, K145Q)

114132  [NCBI]

NKDPSYSLQVQRQVPVPEGLCVIVSCNLSYPRDGWDESTAAYGYWFKGRTSPKTGAPVATNNQSREVAMSTRDRFQLTGDPGKGSCSLVIRDAQREDEAWYFFRVERGSRVRHSFLSNAFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFKKCPAPSFSWTGAALSPRRTRPSTSHFSVLSFTPSPQDHDTDLTCHVDFSRKGVSAQRTVRLRVAYAPKDLIISISHDNTSALELQGNVIYLEVQKGQFLRLLCAADSQPPATLSWVLQDRVLSSSHPWGPRTLGLELRGVRAGDSGRYTCRAENRLGSQQQALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLRLVCVTHSSPPARLSWTRWGQTVGPSQPSDPGVLELPPIQMEHEGEFTCHAQHPLGSQHVSLSLSVHYPPQLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQGSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCKAWNVHGAQSGSVFQLLPGKLEH

Approximately 113-130 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.