Siglec-3/CD33 蛋白, Human (HEK293, hFc)

Siglec-3/CD33 protein is a sialic acid-binding lectin that mediates cell-cell interactions and maintains immune cell quiescence. It prefers α-2,3- and α-2,6-linked glycans with sialic acid. Siglec-3/CD33 Protein, Human (HEK293, hFc) is the recombinant human-derived Siglec-3/CD33 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-3/CD33 Protein, Human (HEK293, hFc) is 242 a.a., with molecular weight (glycosylation form) of 68-80 kDa.

Siglec-3/CD33 蛋白是一种唾液酸结合免疫球蛋白样凝集素，在介导细胞间相互作用和维持免疫细胞处于静息状态方面发挥着至关重要的作用。它表现出对识别和结合 α-2,3- 和更热衷 α-2,6- 连接的唾液酸聚糖的偏好。在与 C1q 或唾液酸化糖蛋白等配体结合后，CD33 胞质尾部内的两个基于免疫受体酪氨酸的抑制基序 (ITIM) 会被 Src 样激酶（如 LCK）磷酸化。这些磷酸化的 ITIM 作为招募和激活蛋白酪氨酸磷酸酶 PTPN6/SHP-1 和 PTPN11/SHP-2 的对接位点，进而通过信号分子的去磷酸化调节下游通路。CD33 对单核细胞活化的抑制作用涉及磷酸肌醇 3-激酶/PI3K。在结构上，该蛋白通过二硫键形成同二聚体，并在磷酸化后与 PTPN6/SHP-1 和 PTPN11/SHP-2 相互作用。它还通过其 C 末端与 C1QA 结合，激活 CD33 抑制基序。

1.Immobilized Human Siglec-3 at 0.5 μg/mL(100 μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC₅₀ of 21.8 ng/mL determined by ELISA.
2.Immobilized Human CD33 at 2 μg/mL (100 μL/well) can bind Anti-CD33 Antibody, the ED₅₀ for this effect is 8.312 ng/mL.

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  Immobilized Human CD33 at 2 μg/mL (100 μL/well) can bind Anti-CD33 Antibody, The ED₅₀ for this effect is 8.312 ng/mL.

Human

HEK293

C-hFc

P20138/AAH28152.1 (D18-H259)

945  [NCBI]

DPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH

68-80 kDa (glycosylation)

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.