SIRP alpha/CD172a 蛋白, Rat (HEK293, His)

When paired with KLRD1, the NKG2C/CD159c protein recognizes MHC-E loaded with MHC-G peptides, thereby contributing to NK cell generation and function. It also regulates cytotoxic lymphocytes in viral infections. SIRP alpha/CD172a Protein, Rat (HEK293, His) is the recombinant rat-derived SIRP alpha/CD172a protein, expressed by HEK293 , with C-His labeled tag. The total length of SIRP alpha/CD172a Protein, Rat (HEK293, His) is 342 a.a., with molecular weight of 63-80 kDa.

SIRP α/CD172a 蛋白是 CD47 的免疫球蛋白样细胞表面受体，在各种细胞过程中充当多功能介质。作为对接蛋白，它诱导 PTPN6、PTPN11 和其他结合配偶体从细胞质易位到质膜。除了在细胞粘附中的作用外，SIRP α 还支持小脑神经元的粘附、神经突生长和神经胶质细胞附着。此外，它在突触发生和突触功能期间的细胞内信号传导中发挥着潜在的关键作用。SIRP α 参与负调节，调节由细胞粘附、生长因子或胰岛素诱导的受体酪氨酸激酶偶联细胞反应。它进一步介导吞噬作用、肥大细胞激活和树突细胞激活的负调节。值得注意的是，CD47 结合可防止未成熟树突细胞的成熟，并抑制成熟树突细胞产生细胞因子。SIRP α 的重要性延伸到抗病毒免疫，因为它通过减少病毒内化来限制新世界沙粒病毒感染。作为 THBS1 的受体，它刺激 SIRPA 的磷酸化，并响应 THBS1，参与非吞噬细胞中的 ROS 信号传导，刺激 NADPH 氧化酶衍生的 ROS 产生。SIRP α 与 PTPN11、GRB2、FGR、JAK2、SCAP1、SCAP2、FYB1、PTK2B 和 TRIM2 等蛋白质进行多种相互作用，说明其在细胞信号传导和免疫反应中作为核心参与者的复杂作用。

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD47 is present at 5 μg/mL, can bind Biotinylated Recombinant Rat CD172a. The ED₅₀ for this effect is 157.1 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD47 is present at 5 μg/mL, can bind Biotinylated Recombinant Rat CD172a .The ED₅₀ for this effect is 157.1 ng/mL.

Rat

HEK293

C-His

P97710 (K32-N373)

25528  [NCBI]

KELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWN

63-80 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.