SIRP beta 1 蛋白, Human (HEK293, His)

SIRP beta 1 protein negatively regulates receptor tyrosine kinase signaling and recruits SYK for myeloid cell activation. It interacts with TYROBP as a homodimer, facilitating SYK recruitment. This intricate molecular interplay is crucial for modulating myeloid cell activation and regulating cellular signaling. SIRP beta 1 occupies a central position in protein interactions that contribute to its functional role. SIRP beta 1 Protein, Human (HEK293, His) is the recombinant human-derived SIRP beta 1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SIRP beta 1 Protein, Human (HEK293, His) is 333 a.a., with molecular weight of 45-55 kDa.

SIRP beta 1 蛋白是一种免疫球蛋白样细胞表面受体，在受体酪氨酸激酶偶联信号传导过程的负调节中发挥着至关重要的作用。此外，它还参与酪氨酸激酶 SYK 的募集，与 TYROBP 相关时触发骨髓细胞的激活。SIRP beta 1 作为具有二硫键的同二聚体存在，与 TYROBP 特异性相互作用，导致 SYK 的募集。这种复杂的分子相互作用将 SIRP beta 1 置于细胞信号传导调节的纽带，突出了其在调节骨髓细胞激活以及有助于其功能作用的复杂蛋白质相互作用网络中的重要性。

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human SIRP beta 1/CD172b at 1 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human SP-D. The ED₅₀ for this effect is 6753 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human SIRP beta 1/CD172b at 1 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human SP-D.The ED₅₀ for this effect is 6753 ng/mL.

Human

HEK293

C-6*His

O00241 (E30-E362)

10326  [NCBI]

EDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHE

45-55 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.