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  3. Sorcin/SRI 蛋白, Human (E.coli, GST)

Sorcin/SRI 蛋白, Human (E.coli, GST)

目录号: HY-P700311
产品使用指南

Sorcin/SRI 蛋白是一种钙结合调节因子,可复杂地调节心脏的兴奋-收缩耦合,并在维持肌浆网内的钙稳态中发挥关键作用。作为同型二聚体,Sorcin/SRI 影响 RYR2 钙通道的活性,有助于精确控制心肌细胞中的钙动态。Sorcin/SRI 蛋白, Human (E.coli, GST) 是重组的 Sorcin/SRI 蛋白,由 E. coli 表达,带有 N-GST 标签。Sorcin/SRI 蛋白, Human (E.coli, GST) 全长 198 个氨基酸,分子量约为 ~48 kDa。

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描述

The Sorcin/SRI protein is a calcium-binding regulator that complexly regulates excitation-contraction coupling in the heart and plays a critical role in maintaining calcium homeostasis within the sarcoplasmic reticulum. As a homodimer, Sorcin/SRI affects the activity of the RYR2 calcium channel, helping to precisely control calcium dynamics in cardiomyocytes. Sorcin/SRI Protein, Human (E.coli, GST) is the recombinant human-derived Sorcin/SRI protein, expressed by E. coli , with N-GST labeled tag. The total length of Sorcin/SRI Protein, Human (E.coli, GST) is 198 a.a., with molecular weight of ~48 kDa.

研究背景

Sorcin (SRI) 是一种钙结合蛋白,对于调节心脏的兴奋-收缩耦合至关重要,在维持肌浆网内的钙稳态方面发挥着重要作用。SRI 已被确定为 RYR2 钙通道的调节剂,表明它参与精细调节细胞内钙动力学。SRI 作为同二聚体存在,还参与蛋白质-蛋白质相互作用,与 GCA、RYR2 和 ANXA7 形成复合物。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

P30626 (M1-V198)

基因 ID
蛋白结构
N-term
GST
SRI (M1-V198)
Accession # P30626
C-term
同用名
22 kDa protein; CP22; V19
分子量

Approximately 48 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/ug, determined by LAL method.

文件资料

Sorcin/SRI Protein, Human (E.coli, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Sorcin/SRI Protein, Human (E.coli, GST)
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HY-P700311
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