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  3. SSB 蛋白, Human (His)

SSB 蛋白, Human (His)

目录号: HY-P73630
产品使用指南

SSB 蛋白与 RNA 聚合酶 III 转录物的 3' Poly(U) 末端关键结合,防止核酸外切酶消化并帮助正确折叠。在柯萨奇病毒 B3 感染中,SSB 与病毒内部核糖体进入位点 (IRES) 结合,增强 IRES 介导的翻译。与 DDX15 的相互作用以及与 RUFY1 的潜在相互作用强调了 SSB 参与多种细胞过程,强调了其多功能性质。SSB 蛋白, Human (His) 是重组的 SSB 蛋白,由 E. coli 表达,带有 N-His 标签。SSB 蛋白, Human (His) 全长 408 个氨基酸,分子量约为 ~47.8 kDa。

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描述

SSB Protein crucially binds to the 3' poly(U) terminus of RNA polymerase III transcripts, safeguarding against exonuclease digestion and aiding proper folding. In Coxsackievirus B3 infection, SSB binds to the viral internal ribosome entry site (IRES), enhancing IRES-mediated translation. Interactions with DDX15 and potential interactions with RUFY1 underscore SSB's involvement in diverse cellular processes, emphasizing its multifunctional nature. SSB Protein, Human (His) is the recombinant human-derived SSB protein, expressed by E. coli, with N-His labeled tag. The total length of SSB Protein, Human (His) is 408 a.a., with molecular weight of ~47.8 kDa.

研究背景

SSB 蛋白通过结合新生 RNA 聚合酶 III 转录物的 3' Poly(U) 末端发挥着至关重要的作用,提供针对核酸外切酶消化的保护并促进其正确折叠和成熟,正如各种研究所证明的那样。此外,在柯萨奇病毒 B3 感染的情况下,SSB 与病毒内部核糖体进入位点 (IRES) 结合,从而刺激 IRES 介导的翻译。该蛋白进一步与 DDX15 相互作用,并可能与 RUFY1 相互作用,表明它参与多种细胞过程并强调其多功能性质。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P05455 (M1-Q408)

基因 ID
蛋白结构
N-term
His
SSB (M1-Q408)
Accession # P05455
C-term
同用名
Lupus La protein; Sjoegren syndrome type B antigen; SS-B
分子量

Approximately 47.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SSB Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HY-P73630
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