SULT2B1 蛋白, Human (364a.a, His)

The SULT2B1 protein uses PAPS to catalyze sulfate conjugation, mainly sulfating cholesterol, and shows significant activity on steroids such as pregnenolone and DHEA. It plays a crucial role in epidermal cholesterol metabolism, regulating epidermal proliferation and differentiation. SULT2B1 Protein, Human (364a.a, His) is the recombinant human-derived SULT2B1 protein, expressed by E. coli , with N-His labeled tag. The total length of SULT2B1 Protein, Human (364a.a, His) is 364 a.a., with molecular weight of ~44 kDa.

SULT2B1 蛋白是一种利用 3'-磷酸-5'-腺苷酸硫酸盐 (PAPS) 作为磺酸盐供体的磺基转移酶，在催化硫酸盐结合中发挥着关键作用，主要负责硫酸化胆固醇。此外，SULT2B1 在硫酸化类固醇方面表现出显着的活性，尤其是孕烯醇酮和脱氢表雄酮 (DHEA) 等分子的 3β-羟基。虽然它表现出胆固醇的优先磺化作用，但它还表现出与孕烯醇酮和 DHEA 的显着活性。除了其酶功能外，SULT2B1 在表皮胆固醇代谢中发挥着至关重要的作用，有助于调节表皮增殖和分化。值得注意的是，它磺化孕烯醇酮，但不磺化胆固醇，强调其底物选择性，并强调其多方面参与控制胆固醇稳态和类固醇代谢的复杂过程。

Measured by its ability to transfer sulfate from PAPS to pregnenolone. The specific activity is ≥15.242 pmol/min/μg that incubate at 37 °C for 30 minutes.

Human

E. coli

N-6*His

O00204-1 (D2-S365)

6820  [NCBI]

DGPAEPQIPGLWDTYEDDISEISQKLPGEYFRYKGVPFPVGLYSLESISLAENTQDVRDDDIFIITYPKSGTTWMIEIICLILKEGDPSWIRSVPIWERAPWCETIVGAFSLPDQYSPRLMSSHLPIQIFTKAFFSSKAKVIYMGRNPRDVVVSLYHYSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMKANTMSNYTLLPPSLLDHRRGAFLRKGVCGDWKNHFTVAQSEAFDRAYRKQMRGMPTFPWDEDPEEDGSPDPEPSPEPEPKPSLEPNTSLEREPRPNSSPSPSPGQASETPHPRPS

Approximately 44 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 10% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.