SUMO3 蛋白, Human (HEK293, His)

SUMO3 is a multifunctional ubiquitin-like protein that can attach to target lysines, acting as a monomer or forming lysine-linked polymers. Unlike ubiquitin, SUMO3 is not involved in protein degradation; instead, it may counteract ubiquitin degradation. SUMO3 Protein, Human (HEK293, His) is the recombinant human-derived SUMO3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SUMO3 Protein, Human (HEK293, His) is 91 a.a., with molecular weight of ~21.0 kDa.

SUMO3 是一种类泛素蛋白，通过共价连接到目标赖氨酸（作为单体或形成赖氨酸连接的聚合物）而表现出多种功能。与泛素不同，SUMO3 似乎不参与蛋白质降解；相反，它可能在降解过程中充当泛素的拮抗剂。它涉及多种细胞过程，包括核运输、DNA 复制和修复、有丝分裂和信号转导。SUMO3 与其底物的共价连接需要由 E1 复合物 SAE1-SAE2 预先激活并与 E2 酶 UBE2I 连接，并由 E3 连接酶（例如 PIAS1-4、RANBP2 或 CBX4）促进促进。SUMO3 参与 SETX 苏酰化状态的调节，并与多种蛋白质相互作用，包括 BMAL1、USP25（通过其 SIM 结构域）、SAE2 和 UBE2I。值得注意的是，它与 USP25 的相互作用导致 USP25 的苏酰化，抑制其泛素水解活性。

Human

HEK293

C-6*His

P55854 (S2-G92)

6612  [NCBI]

SEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG

Approximately 21.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 10% Trehalose, 3% Dextran-70, 50 mM NaCl, 0.05% Tween 80, pH 3.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.