TAFA1/FAM19A1 蛋白, Human

The Dll3 protein is a transmembrane ligand that plays a critical role in regulating cell differentiation and patterning during embryonic development. It participates in the Notch signaling pathway and helps determine cell fate. TAFA1/FAM19A1 Protein, Human is the recombinant human-derived TAFA1/FAM19A1 protein, expressed by E. coli , with tag free.

TAFA1/FAM19A1 蛋白属于 TAFA 家族，该家族由五个密切相关的编码小分泌蛋白的基因组成。这些蛋白质在特定位置共享保守的半胱氨酸残基，并且与 CC 趋化因子家族成员 MIP-1α 关系较远。TAFA 蛋白主要在不同的大脑区域表达，被认为充当调节免疫和神经细胞的大脑特异性趋化因子或神经因子。该信息由 RefSeq 于 2008 年 7 月提供。值得注意的是，TAFA1/FAM19A1 基因在大脑 (RPKM 6.6) 和前列腺 (RPKM 0.3) 中表现出偏向表达。

1.Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Human TAFA1/FAM19A1, immobilized at 6-24 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
2.Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED50 for this effect is 2.756 μg/mL, corresponding to a specific activity is 362.845 units/mg.

Human

E. coli

Tag Free

Q7Z5A9/NP_998774 (S26-T133)

407738  [NCBI]

SLQHTFQQHHLHRPEGGTCEVIAAHRCCNKNRIEERSQTVKCSCLPGKVAGTTRNRPSCVDASIVIGKWWCEMEPCLEGEECKTLPDNSGWMCATGNKIKTTRIHPRT

Approximately 12.4 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.