Thymosin beta 4 蛋白, Human

TMSB4X proteins play a critical role in cytoskeletal organization, influencing actin dynamics by binding and sequestering actin monomers. As a potent inhibitor of actin polymerization, it affects the cytoskeleton. Thymosin beta 4 Protein, Human is the recombinant human-derived Thymosin beta 4 protein, expressed by E. coli , with tag free. The total length of Thymosin beta 4 Protein, Human is 43 a.a., with molecular weight of ~4.9 kDa.

TMSB4X 蛋白在细胞骨架组织中发挥着至关重要的作用，其对肌动蛋白动力学的影响已被证实。它通过结合和隔离肌动蛋白单体（G 肌动蛋白）发挥作用，从而充当肌动蛋白聚合的有效抑制剂。除了对细胞骨架的影响之外，TMSB4X 还作为骨髓源性干细胞分化的强力抑制剂，通过阻止造血多能干细胞进入 S 期来发挥其作用。这些多方面的功能强调了 TMSB4X 在协调基本细胞过程（包括细胞骨架完整性和干细胞分化）中的重要性，强调了其在维持细胞稳态和协调动态细胞反应中的调节作用。

1.The biological activity determined by its ability to produce a protective effect against hydrogen peroxide in primary lung fibroblasts is in a concentration range of 0.5 - 10 μg/mL.
2.Measured in inhibiting cell proliferation assay using A549 cells. The ED₅₀ for this effect is 1.824 μg/mL.

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  Measured in inhibiting cell proliferation assay using A549 cells. The ED₅₀ for this effect is 1.824 μg/mL, corresponding to a specific activity is 5.482×10² units/mg.

Human

E. coli

Tag Free

P62328 (S2-S44)

7114  [NCBI]

SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES

Approximately 12 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg; determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.