TIM-4/TIMD-4 蛋白, Human (HEK293, His)

T-cell immunoglobulin and mucin domain-containing protein 4 (TIMD4, TIM4) is a membrane protein in TIM family and is a phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. TIMD4 controls T-cell activation in a bimodal fashion and promots the engulfment against apoptotic cells or exogenous particles. TIM-4/TIMD-4 Protein, Human (HEK293, His) is the recombinant human-derived TIM-4/TIMD-4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TIM-4/TIMD-4 Protein, Human (HEK293, His) is 291 a.a., with molecular weight of 60-90 kDa.

T 细胞免疫球蛋白和粘蛋白结构域蛋白 4 (TIMD4, TIM4) 是 TIM 家族中的一个膜蛋白，是一种磷脂酰丝氨酸受体，在凋亡细胞的吞噬和 T 细胞调节等免疫应答中发挥不同的作用。TIMD4 双模式地控制 T 细胞的激活，通过诱导细胞周期阻滞来降低初始 T 细胞的激活，同时通过激活 AKT1 和 ERK1/2 磷酸化以及随后的信号通路来增加活化 T 细胞的增殖。TIMD4 还在吞噬细胞清除凋亡细胞的胞葬作用中发挥作用，通过与凋亡细胞上存在的磷脂酰丝氨酸直接结合，将凋亡细胞或外源颗粒固定在吞噬细胞上，从而促进凋亡细胞或外源颗粒的吞噬，而其他吞噬受体如 MERTK 则能有效识别凋亡细胞并介导其摄取。TIMD4 还通过磷脂酰丝氨酸依赖机制激活 LKB1/PRKAA1 通路，抑制 NLRP3 炎性体活性，从而促进自噬过程^[1][2][3][4]。

Human

HEK293

C-6*His

AAH08988.1 (E25-L315)

91937  [NCBI]

ETVVTEVLGHRVTLPCLYSSWSHNSNSMCWGKDQCPYSGCKEALIRTDGMRVTSRKSAKYRLQGTIPRGDVSLTILNPSESDSGVYCCRIEVPGWFNDVKINVRLNLQRASTTTHRTATTTTRRTTTTSPTTTRQMTTTPAALPTTVVTTPDLTTGTPLQMTTIAVFTTANTCLSLTPSTLPEEATGLLTPEPSKEGPILTAESETVLPSDSWSSAESTSADTVLLTSKESKVWDLPSTSHVSMWKTSDSVSSPQPGASDTAVPEQNKTTKTGQMDGIPMSMKNEMPISQL

60-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.