TMEM173 蛋白, Human (Sumo-His)

The TMEM173 protein acts as a promoter of innate immune signaling, acting as a sensor of bacterial and viral cytoplasmic DNA, ultimately promoting the production of type I interferons (IFN-α and IFN-β). This innate immune response is triggered in response to the delivery of non-CpG double-stranded DNA from viruses and bacteria into the cytoplasm. TMEM173 Protein, Human (Sumo-His) is the recombinant human-derived TMEM173 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of TMEM173 Protein, Human (Sumo-His) is 187 a.a., with molecular weight of 35-38 kDa.

TMEM173 蛋白充当先天免疫信号传导的促进剂，充当细菌和病毒胞质 DNA 的传感器，最终促进 I 型干扰素（IFN-α 和 IFN-β）的产生。这种先天免疫反应是对来自病毒和细菌的非 CpG 双链 DNA 传递到细胞质的反应而触发的。TMEM173 识别并结合环状二核苷酸，特别是环状二 GMP (c-di-GMP)（细菌产生的第二信使）和环状 GMP-AMP (cGAMP)（CGAS 响应细胞质中的 DNA 病毒而产生的信使）。与 c-di-GMP 或 cGAMP 结合后，TMEM173 寡聚化，从内质网移位，并被 TBK1 磷酸化，导致转录因子 IRF3 的募集和激活。这会诱导 I 型干扰素的表达，建立有效的抗病毒状态。此外，TMEM173 在自噬中发挥直接作用，cGAMP 结合启动 ERGIC 形成，ERGIC 作为自噬体形成的膜源，靶向胞质 DNA 或 DNA 病毒进行溶酶体降解。TMEM173 的多方面活性凸显了其在协调先天免疫反应和自噬过程中的核心作用，有助于细胞防御病毒和细菌威胁。

Human

E. coli

N-SUMO;N-6*His

Q86WV6 (V155-V341)

340061  [NCBI]

VAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEV

35-38 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 100 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.