TNIK 蛋白, Human (Sf9)

TNIK protein is a key activator of the Wnt signaling pathway and regulates gene expression by phosphorylating TCF4/TCF7L2 at the promoter of Wnt target genes. TNIK is located upstream of the JUN N-terminal pathway and contributes to the complex Wnt signaling cascade. TNIK Protein, Human (Sf9) is the recombinant human-derived TNIK protein, expressed by Sf9 insect cells , with tag free. The total length of TNIK Protein, Human (Sf9) is 304 a.a., .

TNIK 是一种丝氨酸/苏氨酸激酶，是 Wnt 信号通路的关键激活剂，在基因表达调控中发挥着至关重要的作用。它的参与延伸到 Wnt 靶基因启动子处的 TCF4/TCF7L2 磷酸化，从而促进它们的激活。TNIK 位于 JUN N 末端通路的上游，有助于 Wnt 信号传导的复杂级联。除了其典型功能之外，TNIK 还参与对环境压力的反应，并构成信号复合体的一部分，该信号复合体由 NEDD4、RAP2A 和 TNIK 组成，在发育过程中协调神经元树突延伸和分枝化。在更广泛的背景下，TNIK 在细胞骨架重排和细胞扩散调节中表现出潜在作用。值得注意的是，它通过磷酸化 SMAD1 Thr-322 来发挥影响，从而扩展其在多种细胞过程中的调节能力。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.

Human

Sf9 insect cells

Tag Free

Q9UKE5 (D11-G314)

23043

34.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 10% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.