TRXR1/TXNRD1 蛋白, Human (N-His)

The homodimeric flavoprotein TRXR1/TXNRD1 plays a key role in cellular redox regulation, growth and differentiation. It reduces the disulfide protein thioredoxin (Trx) to its dithiol-containing form and exhibits reductase activity towards hydrogen peroxide (H2O2). TRXR1/TXNRD1 Protein, Human (N-His) is the recombinant human-derived TRXR1/TXNRD1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TRXR1/TXNRD1 Protein, Human (N-His) is 487 a.a., with molecular weight of ~60 kDa.

TRXR1/TXNRD1 蛋白作为同二聚黄素蛋白发挥作用，通过将二硫键蛋白硫氧还蛋白 (Trx) 还原为其含二硫醇的形式，对调节细胞氧化还原反应、生长和分化至关重要。该蛋白质在 C 端活性位点含有催化必需的硒代半胱氨酸残基，对过氧化氢 (H2O2) 表现出还原酶活性。除了其氧化还原调节作用外，TRXR1/TXNRD1 还诱导肌动蛋白和微管蛋白聚合，有助于细胞膜突起的形成。这些多方面的活动强调了它在细胞过程中的重要性，强调了它在维持氧化还原稳态和影响细胞形态和信号传导的关键方面的参与。

Human

E. coli

N-6*His

Q16881 (Y161-C647)

7296  [NCBI]

YDYDLIIIGGGSGGLAAAKEAAQYGKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQAGC

Approximately 60 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.