TSPAN1 蛋白, Human (HEK293, His)

TSPAN1 Protein is a member of the tetraspanin (TSPAN/TM4SF) family of proteins whose important feature is their ability to aggregate with one another or various other transmembrane receptors, to become TSPAN-enriched microdomains (TEMs). TSPAN1 functions as an oncoprotein in many cancer types, especially digestive malignancies such as hepatocellular carcinoma (HCC), pancreatic, gastric, colorectal, and esophageal cancers. TSPAN1 is a novel positive regulator of macroautophagy/autophagy characterized by decreased LC3-II and SQSTM1/p62 expressions, inhibited puncta formation of GFP-LC3 and autophagic vacuoles^[1][2][3]. TSPAN1 Protein, Human (HEK293, His) is the recombinant human-derived TSPAN1 protein, expressed by HEK293 , with N-His labeled tag. The total length of TSPAN1 Protein, Human (HEK293, His) is 102 a.a., with molecular weight of ~32 & 27 & 18 & 14 kDa, respectively.

TSPAN1 在胰腺癌中表达上调，并且 TSPAN1 缺失会降低体外和体内胰腺癌细胞的增殖。TSPAN1 通过两个保守的 LIR 基序直接与 LC3 结合，促进自噬成熟。TSPAN1 LIR 基序的突变导致诱导自噬和促进胰腺癌增殖的能力丧失^[1]。
亲代和 CDDP 耐药 HNSCC 细胞中 TSPAN1 的缺失会减少细胞增殖、诱导细胞凋亡、减少自噬、对化疗药物敏感并抑制多种信号级联反应，其中磷酸化 SRC 抑制是主要的靶标^[2]。

Human

HEK293

N-His

O60635 (Y110-N211)

10103  [NCBI]

YTTMAEHFLTLLVVPAIKKDYGSQEDFTQVWNTTMKGLKCCGFTNYTDFEDSPYFKENSAFPPFCCNDNVTNTANETCTKQKAHDQKVEGCFNQLLYDIRTN

Approximately 13 kDa & 19-32 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.