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  4. TUBB4A 蛋白, Human (His)

TUBB4A 蛋白, Human (His)

目录号: HY-P71075
产品使用指南

TUBB4A 蛋白是微管的主要成分，通过 α 和 β 微管蛋白原丝的横向缔合形成圆柱形结构。GTP-微管蛋白二聚体促进微管生长，形成稳定帽。在此之下，TUBB4A 二聚体转变为 GDP 结合状态，受 α-微管蛋白的 GTP 酶活性调节。这种复杂的机制凸显了 TUBB4A 在控制微管组装和稳定性方面的关键作用。TUBB4A 蛋白, Human (His) 是重组的 TUBB4A 蛋白，由 E. coli 表达，带有 N-6*His 标签。TUBB4A 蛋白, Human (His) 全长 444 个氨基酸，分子量约为 ~58.0 kDa。

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描述

TUBB4A protein, the main constituent of microtubules, forms cylindrical structures via lateral association of alpha- and beta-tubulin protofilaments. Microtubule growth, facilitated by GTP-tubulin dimers, leads to a stabilizing cap. Below this, TUBB4A dimers transition to a GDP-bound state, regulated by alpha-tubulin's GTPase activity. This intricate mechanism highlights TUBB4A's crucial role in governing microtubule assembly and stability. TUBB4A Protein, Human (His) is the recombinant human-derived TUBB4A protein, expressed by E. coli , with N-6*His labeled tag. The total length of TUBB4A Protein, Human (His) is 444 a.a., with molecular weight of ~58.0 kDa.
研究背景

TUBB4A 蛋白作为微管的主要成分占据中心地位，通过由 α 和 β 微管蛋白异二聚体组成的线性原丝的横向缔合，有助于形成圆柱形结构。通过在微管末端添加 GTP-微管蛋白二聚体促进微管的动态生长，从而形成稳定帽。低于此上限，TUBB4A 蛋白二聚体转变为 GDP 结合状态，这一过程受 α-微管蛋白的 GTP 酶活性调节。这种复杂的机制强调了 TUBB4A 在控制微管的组装和稳定性方面的关键作用。
种属

Human
表达系统

E. coli
标签

N-6*His
蛋白编号

P04350 (M1-A444)
基因 ID
蛋白结构
N-term
6*His
TUBB4A (M1-A444)
Accession # P04350
C-term
同用名
Tubulin Beta-4A Chain; Tubulin 5 Beta; Tubulin Beta-4 Chain; TUBB4A; TUBB4; TUBB5
氨基酸序列

MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEGEFEEEAEEEVA

分子量

Approximately 58.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TUBB4A Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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