1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E2 Enzymes
  5. UBE2N/Ubc13 蛋白, Human (sf9, His, StrepⅡ)

UBE2N/Ubc13 蛋白, Human (sf9, His, StrepⅡ)

目录号: HY-P79452
产品使用指南

UBE2F 蛋白接受来自 UBA3-NAE1 E1 复合物的 NEDD8,并将其与泛素样 NEDD8 缀合途径中的各种靶蛋白共价连接。RBX2-UBE2F 复合物与 E3 泛素连接酶 RBX2 特异性相互作用,但不与 RBX1 相互作用,以实现特定靶标(尤其是 CUL5)的 neddylation。UBE2N/Ubc13 蛋白, Human (sf9, His, Strep) 是重组的 UBE2N/Ubc13 蛋白,由 Sf9 insect cells 表达,带有 N-8*His, N-Strep 标签。UBE2N/Ubc13 蛋白, Human (sf9, His, Strep) 全长 151 个氨基酸。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The UBE2F protein accepts NEDD8 from the UBA3-NAE1 E1 complex and covalently links it to various target proteins in the ubiquitin-like NEDD8 conjugation pathway. The RBX2-UBE2F complex specifically interacts with the E3 ubiquitin ligase RBX2, but not RBX1, for the neddylation of specific targets, especially CUL5. UBE2N/Ubc13 Protein, Human (sf9, His, Strep) is the recombinant Rhesus Macaque, cynomolgus-derived UBE2N/Ubc13 protein, expressed by Sf9 insect cells , with N-8*His, N-Strep labeled tag. The total length of UBE2N/Ubc13 Protein, Human (sf9, His, Strep) is 151 a.a., .

研究背景

UBE2F 蛋白在泛素样蛋白 NEDD8 缀合途径中发挥着至关重要的作用,从 UBA3-NAE1 E1 复合物中接受 NEDD8 并催化其与各种靶蛋白的共价附着。它与 E3 泛素连接酶 RBX2(而不是 RBX1)的独特相互作用意味着 RBX2-UBE2F 复合物专门用于特定靶蛋白(尤其是 CUL5)的 neddylation。这种特定的相互作用和 neddylation 活性凸显了 UBE2F 参与与关键底物的靶向泛素化相关的细胞过程的调节。

种属

Human

表达系统

Sf9 insect cells

标签

N-StrepⅡ;N-8*His

蛋白编号

P61088 (A2-I152)

基因 ID
蛋白结构
N-term
8*His-StrepⅡ
UBE2N (A2-I152)
Accession # Q969M7
C-term
同用名
Ubiquitin-conjugating enzyme E2 N; UbcH13
纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UBE2N/Ubc13 Protein, Human (sf9, His, StrepⅡ) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
UBE2N/Ubc13 Protein, Human (sf9, His, StrepⅡ)
目录号:
HY-P79452
需求量: