UCHL3 蛋白, Human (His)

UCHL3 Protein, a ubiquitin carboxyl-terminal hydrolase, is responsible for protein degradation and regulation of cellular processes. It is prominently expressed in the testes and plays a crucial role in spermatogenesis. UCHL3 Protein's involvement in male fertility and its potential as a therapeutic target in reproductive disorders make it a subject of interest in reproductive medicine. UCHL3 Protein, Human (His) is the recombinant human-derived UCHL3 protein, expressed by E. coli , with C-6*His labeled tag. The total length of UCHL3 Protein, Human (His) is 230 a.a., with molecular weight of ~25.0 kDa.

UCHL3 蛋白是一种去泛素化酶 (DUB)，通过处理泛素前体和水解泛素化蛋白，在控制细胞泛素水平方面发挥着至关重要的作用。作为硫醇蛋白酶，UCHL3 对 P3'' 位的 Arg 和 Lys 表现出 10 倍的偏好，对“Lys-48”连接的泛素链具有特殊的亲和力。在顶室中，UCHL3 使 ENAC 去泛素化，影响顶膜回收。此外，它还间接增强 IGFIR、AKT 和 FOXO1 的磷酸化，促进胰岛素信号传导和胰岛素诱导的脂肪生成。UCHL3 的参与延伸到视网膜、骨骼肌和生殖细胞的应激反应，有助于它们的维持。值得注意的是，它可以水解 UBB(+1)，这是一种与神经退行性疾病相关的泛素突变形式，它不能被蛋白酶体有效降解。这种多方面的酶也可能在工作记忆中发挥作用。

Human

E. coli

C-6*His

P15374 (M1-A230)

7347  [NCBI]

MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA

Approximately 25.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 50% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.