UCHL1 蛋白, Mouse (His)

UCHL1 Protein, a ubiquitin carboxyl-terminal hydrolase, plays a crucial role in protein degradation and regulation of cellular processes.It is involved in maintaining neuronal integrity and has been linked to neurodegenerative diseases.UCHL1 Protein's potential as a biomarker and therapeutic target in neurodegenerative disorders makes it a subject of intense research in the field of neuroscience.UCHL1 Protein, Mouse (His) is the recombinant mouse-derived UCHL1 protein, expressed by E.coli , with N-His labeled tag.

UCHL1 蛋白是一种去泛素酶，在多种过程中发挥着至关重要的作用，包括维持突触功能、心脏功能、炎症反应和破骨细胞生成。它抑制多种蛋白质的泛素化，例如 WWTR1/TAZ、EGFR、HIF1A 和 β 位点淀粉样蛋白前体蛋白裂解酶 1/BACE1。此外，它还能识别并水解泛素 C 端甘氨酸的肽键，这有助于维持泛素-蛋白酶体和自噬-溶酶体途径所需的稳定的单泛素池。UCHL1 蛋白还通过促进 BACE1 降解来调节淀粉样蛋白前体蛋白/APP 加工，从而减少淀粉样蛋白 β 的产生。它通过调节 MHC I 分子到达生成 Ag-MHC I 复合物所必需的隔室的能力来参与免疫反应。此外，UCHL1 蛋白介导转录共激活因子 WWTR1/TAZ 的去泛素化，使其稳定并抑制破骨细胞生成。它还可以稳定表皮生长因子受体 EGFR，防止其降解并激活下游介质。此外，UCHL1 蛋白通过调节关键线粒体氧化蛋白来调节骨骼肌的氧化活性。最后，它通过废除 VHL E3 连接酶介导的泛素化来抑制缺氧诱导因子 1-α/HIF1A 的降解，从而增强缺氧诱导因子 1-α/HIF1A 的活性。

Measured by the hydrolysis of Ubiquitin-AMC that incubate at 37°C for 10 min. The specific activity is 1906.363 pmol/min/μg.

Mouse

E. coli

N-6*His

Q9R0P9 (Q2-A223)

22223  [NCBI]

QLKPMEINPEMLNKVLAKLGVAGQWRFADVLGLEEETLGSVPSPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLEFEDGSVLKQFLSETEKLSPEDRAKCFEKNEAIQAAHDSVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDSLLQDAAKVCREFTEREQGEVRFSAVALCKAA

Approximately 27 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, 20% Glycerol, pH 7.7.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.