USP5 蛋白, Human (sf9, His)

USP5 protein selectively cleaves branched multiubiquitin polymers, favoring 'Lys-48'-linked polyubiquitin disassembly. It exhibits lower affinity for linear and 'Lys-63'-linked polyubiquitin. USP5 knockdown leads to p53/TP53 accumulation, enhancing p53/TP53 transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition, unlike MDM2. USP5 Protein, Human (sf9, His) is the recombinant human-derived USP5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of USP5 Protein, Human (sf9, His) is 835 a.a., with molecular weight of ~100 kDa.

USP5 是一种去泛素化酶，表现出对切割线性和支链多泛素聚合物的明显偏好，对支化结构具有特殊的亲和力。它积极参与未锚定“Lys-48”连接的多聚泛素链的分解。虽然 USP5 以相对较低的亲和力结合线性和“Lys-63”连接的多聚泛素，但其敲低会导致 p53/TP53 的积累和 p53/TP53 转录活性的升高。这种效应归因于在没有 USP5 的情况下，未锚定的多聚泛素能够与泛素化的 p53/TP53 竞争，但不与 MDM2 竞争蛋白酶体的识别。

Human

Sf9 insect cells

C-His

P45974-2 (M1-S835)

8078  [NCBI]

Approximately 100 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.