USP7 蛋白, Human (His)

The USP7 protein is a hydrolase that deubiquitinates multiple targets, including FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5, and DAXX. It cooperates with DAXX to enhance the E3 ligase activity of MDM2 and promote ubiquitination and degradation of p53/TP53. USP7 Protein, Human (His) is the recombinant human-derived USP7, expressed by E. coli , with N-His labeled tag. ,

USP7 蛋白作为水解酶，在多种靶蛋白去泛素化中发挥着关键作用，包括 FOXO4、DEPTOR、KAT5、p53/TP53、MDM2、ERCC6、DNMT1、UHRF1、PTEN、KMT2E/MLL5 和 DAXX。它与 DAXX 合作，防止 MDM2 自身泛素化，并增强 MDM2 对 p53/TP53 的 E3 连接酶活性，促进 p53/TP53 泛素化和随后的降解。此外，USP7 参与早期胚胎发育过程中的细胞增殖、响应紫外线损伤的转录偶联核苷酸切除修复 (TC-NER) 以及 DNA 甲基化的维持。它调节染色质结构，表现出对“Lys-48”连接的泛素链的偏好，并调节生物钟。此外，USP7 有助于稳定参与糖异生、神经祖细胞维持和内体表面肌动蛋白聚合的特定蛋白质。重要的是，它的多样化功能凸显了它在细胞过程和微生物感染反应中的重要性，例如它在 HSV-1 感染期间参与疱疹病毒 1 反式作用转录蛋白 ICP0/VMW110 的稳定和反式激活。

The fundamental role of USP7 is specific removal of ubiquitin from substrates. USP7 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

E. coli

N-His

Q93009 (K208-E560)

7874

42.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 10 mM Tris, 150 mM NaCl, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.