Vaspin 蛋白, Human (HEK293, His)

Vaspin is an adipokine that regulates adipogenesis, metabolism, and inflammation by binding to the chemokine receptors CMKLR1 and CMKLR2. It mainly regulates adipocyte differentiation and affects lipid and glucose metabolism genes. Vaspin Protein, Human (HEK293, His) is the recombinant human-derived Vaspin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Vaspin Protein, Human (HEK293, His) is 394 a.a., with molecular weight of 50-60 kDa.

Chemerin/RARRES2 是一种脂肪细胞分泌蛋白（脂肪因子），通过激活趋化因子样受体 1 (CMKLR1) 并充当 CMKLR2 的配体，对脂肪生成、代谢和炎症产生多方面的影响。虽然它可以以较低的亲和力与 CC 趋化因子受体样 2 (CCRL2) 结合，但其主要作用包括正向调节脂肪细胞分化并影响与脂质和葡萄糖代谢相关的基因的表达。此外，Chemerin/RARRES2 在血管生成中发挥着关键作用，这对于白色脂肪组织的扩张至关重要。作为一种促炎性脂肪因子，它会放大促炎性和促糖尿病性脂肪因子的分泌，导致代谢功能受损和全身效应，例如胰岛素敏感性改变、葡萄糖和脂质代谢紊乱以及各种组织中的血管功能受损。它被不同的蛋白酶裂解可以赋予促炎和抗炎特性。作为趋化因子，它吸引表达 CMKLR1 的白细胞群，包括浆细胞样树突状细胞、髓样树突状细胞、巨噬细胞和自然杀伤细胞。Chemerin/RARRES2 还通过抑制 TNF/TNFA 诱导的 VCAM1 表达和血管内皮细胞中的单核细胞粘附来发挥抗炎作用。这种作用是通过抑制 NF-kappa-B 和 CRK/p38 激活来介导的，涉及刺激 AKT1/NOS3 信号传导和一氧化氮的产生。这种炎症和代谢的双重作用表明慢性炎症和肥胖相关疾病（例如 2 型糖尿病和心血管疾病）之间存在潜在联系。此外，Chemerin/RARRES2 还展示了皮肤抗菌功能。

Measured by its ability to inhibit KLK7 cleavage 10 μM Mca-RPKPVE-Nval-WRK(Dnp)-NH2(HY-P2185A ) that incubate at 37°C for 2 hours. The IC₅₀ value is 11.04 nM.

Human

HEK293

C-10*His

Q8IW75 (L21-K414)

145264  [NCBI]

LKPSFSPRNYKALSEVQGWKQRMAAKELARQNMDLGFKLLKKLAFYNPGRNIFLSPLSISTAFSMLCLGAQDSTLDEIKQGFNFRKMPEKDLHEGFHYIIHELTQKTQDLKLSIGNTLFIDQRLQPQRKFLEDAKNFYSAETILTNFQNLEMAQKQINDFISQKTHGKINNLIENIDPGTVMLLANYIFFRARWKHEFDPNVTKEEDFFLEKNSSVKVPMMFRSGIYQVGYDDKLSCTILEIPYQKNITAIFILPDEGKLKHLEKGLQVDTFSRWKTLLSRRVVDVSVPRLHMTGTFDLKKTLSYIGVSKIFEEHGDLTKIAPHRSLKVGEAVHKAELKMDERGTEGAAGTGAQTLPMETPLVVKIDKPYLLLIYSEKIPSVLFLGKIVNPIGK

50-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.