VISTA/B7-H5 蛋白, Mouse (HEK293, Fc)

VISTA/B7-H5 Protein is a type I transmembrane protein expressed primarily in white blood cells that inhibits T cell function. VISTA/B7-H5 Protein promotes embryonic stem cell differentiation by inhibiting BMP4 signaling and stimulates MMP14 mediated MMP2 activation. VISTA/B7-H5 Protein is highly expressed in tumors. VISTA/B7-H5 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived VISTA/B7-H5 protein, expressed by HEK293 , with C-hFc labeled tag.

VISTA 是一种 I 型跨膜蛋白具有免疫检查点的功能。VISTA 是 B7 家族的一员，主要在白细胞中表达，其转录部分受 p53 控制。VISTA 可以作为 T 细胞的配体和受体，抑制 T 细胞效应功能，维持外周耐受性。VISTA 通过抑制 BMP4 信号传导促进胚胎干细胞分化。VISTA 刺激 MMP14 介导的 MMP2 激活。VISTA 在肿瘤浸润淋巴细胞中高水平产生，在黑色素瘤和鳞状细胞癌小鼠模型中，用抗体阻断 VISTA 可导致肿瘤生长延迟。VISTA 水平的增加与免疫激活的增加和 CD4-阳性 T 细胞的减少相关。

Mouse

HEK293

C-hFc

Q9D659 (F33-A194)

74048  [NCBI]

FKVTTPYSLYVCPEGQNATLTCRILGPVSKGHDVTIYKTWYLSSRGEVQMCKEHRPIRNFTLQHLQHHGSHLKANASHDQPQKHGLELASDHHGNFSITLRNVTPRDSGLYCCLVIELKNHHPEQRFYGSMELQVQAGKGSGSTCMASNEQDSDSITAAALA

60-68 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.