WDR77 蛋白, Human (His-SUMO)

WDR77 is a non-catalytic component of the methylosome complex with PRMT5 and CLNS1A and regulates arginine to dimethylarginine in spliceosomal Sm proteins and histones. This modification directs Sm proteins to SMN complexes, helping them assemble into small nuclear ribonucleoprotein core particles. WDR77 Protein, Human (His-SUMO) is the recombinant human-derived WDR77 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of WDR77 Protein, Human (His-SUMO) is 312 a.a., with molecular weight of ~49.6 kDa.

WDR77 作为甲基小体复合物中的非催化成分，与 PRMT5 和 CLNS1A 一起，协调剪接体 Sm 蛋白和组蛋白中特定精氨酸向二甲基精氨酸的修饰。这种修饰引导 Sm 蛋白促进运动神经元 (SMN) 复合物的存活，促进它们组装成小的核核糖核蛋白核心颗粒。除了在 RNA 加工中的作用之外，WDR77 还可能在转录调控中发挥作用。甲基化复合体将其甲基化活性延伸至 Piwi 蛋白（PIWIL1、PIWIL2 和 PIWIL4），这对于它们与包含 Tudor 结构域的蛋白相互作用以及随后定位到减数分裂核至关重要。WDR77 与 PRMT1、ERH 和 CLNS1A 等其他成分发生复杂的相互作用，并观察到与 PRMT5、各种 Sm 蛋白、SUZ12、组蛋白 H2A、CTDP1、LSM11、APEX1、AR、NKX3-1、CHTOP、FAM47E、和 TSC22D2。其复杂的相互作用网络强调了 WDR77 在表观遗传修饰和调控途径中的多方面参与。

Human

E. coli

N-His;N-SUMO

Q9BQA1 (1M-312L)

79084  [NCBI]

MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSL

Approximately 51 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.