ZBTB17 蛋白, Human (His)

The ZBTB17 protein is a multifunctional transcription factor that functions as a binding partner-based activator or repressor and a targeted regulator of cell cycle progression. It is essential for early lymphocyte development, preventing apoptosis and ensuring lineage commitment. ZBTB17 Protein, Human (His) is the recombinant human-derived ZBTB17 protein, expressed by E. coli , with N-6*His labeled tag. The total length of ZBTB17 Protein, Human (His) is 188 a.a., with molecular weight of ~18.0-26.0 kDa.

ZBTB17 是一种多功能转录因子，根据其结合伙伴及其细胞周期进程的目标负调节因子发挥激活剂或阻抑剂的作用。它在早期淋巴细胞发育中发挥着至关重要的作用，防止淋巴前体细胞凋亡，使细胞能够响应 IL7 而存活，并确保正确的谱系定型。ZBTB17 已被鉴定与腺病毒主要晚期蛋白和细胞周期蛋白 D1 的启动子结合，激活转录。ZBTB17 对于原肠胚形成过程中的早期胚胎发育至关重要，它还抑制 RB1 转录，这种作用通过与 ZBTB49 同工型 3/ZNF509S1 的相互作用而抵消。通过 DNA 结合所需的同源寡聚化，ZBTB17 与 GIF1 相互作用，导致 MYB 募集到 CDKN1A/p21 和 CDKN1B 启动子，从而抑制转录。此外，它与 TRAF2 相互作用，抑制 TRAF2 E3 连接酶活性，并与 MYC 结合，使 ZBTB17 不溶于细胞核并抑制其反式激活和生长停滞活性。与 HCFC1、MAGEA4、TMPRSS11A 和 BCL6 的其他相互作用进一步说明了 ZBTB17 在细胞过程中精心策划的复杂调控网络。

Human

E. coli

N-6*His

Q13105 (M1-A188)

7709  [NCBI]

MDFPQHSQHVLEQLNQQRQLGLLCDCTFVVDGVHFKAHKAVLAACSEYFKMLFVDQKDVVHLDISNAAGLGQVLEFMYTAKLSLSPENVDDVLAVATFLQMQDIITACHALKSLAEPATSPGGNAEALATEGGDKRAKEEKVATSTLSRLEQAGRSTPIGPSRDLKEERGGQAQSAASGAEQTEKADA

Approximately 18.0-26.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.