ZYX 蛋白, Human (HEK293, His)

ZYX protein is a focal adhesion protein that binds α-actin and CRP to target TES and ENA/VASP to focal adhesions to form actin-rich structures. It may play a crucial role in adhesion-stimulated gene expression. ZYX Protein, Human (HEK293, His) is the recombinant human-derived ZYX protein, expressed by HEK293 , with N-6*His labeled tag. The total length of ZYX Protein, Human (HEK293, His) is 571 a.a., with molecular weight of ~65.1 kDa.

ZYX 蛋白作为粘附斑蛋白，在结合 α-辅肌动蛋白和 CRP 蛋白方面发挥着关键作用。其意义在于将 TES 和 ENA/VASP 家族成员靶向粘着斑并有助于形成富含肌动蛋白的结构。ZYX 可能是信号转导途径的重要组成部分，该途径协调粘附刺激的基因表达变化。值得注意的是，它与 HPV 6 型蛋白 E6 相互作用，同时与 HPV 11、16 或 18 型的 E6 蛋白相互作用有限。ZYX 富含 Pro 的区域与 ENAH、EVL 和 VASP 的 EVH1 结构域相互作用，并且它还与 TES、NEBL（异构体 2）和 SYNPO2 的第一个 LIM 结构域相互作用，强调了其多样化的分子关联及其在介导细胞信号传导事件中的作用。

Human

HEK293

N-6*His

Q15942 (A2-T572)

7791  [NCBI]

AAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT

Approximately 65.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.