Subtilisin, bacillus licheniformis  (Synonyms: Bacillopeptidaseb)

Subtilisin (Compound proteinase) (EC 3.4.21.62) is a proteolytic enzyme, isolated from Bacillus licheniformis. Subtilisin (Compound proteinase) has catalytic activity in anhydrous dimethyl formamide. Subtilisin (Compound proteinase) can be used as a catalyst for easy coupling between sugars and amino acids^[1][2].

Subtilisin (Compound proteinase) (30 mg/mL；2.5-7 d) 在无水二甲基甲酰胺中选择性的酰化二糖^[1]。
Subtilisin (Compound proteinase) (1.8 g；20-72 h) 在无水二甲基甲酰胺中选择性的酰化单糖^[1]。
Subtilisin (Compound proteinase) 在活化的丁酸三氟乙酯 (TFEB) 存在下，可以酰化黄烷醇异槲皮素 (1) 的 D-葡萄糖^[3]。
Subtilisin (Compound proteinase) 改善了蛋白质和脂肪酸的溶解度，恢复了多肽和不饱和脂肪酸^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Subtilisin (Compound proteinase) 水解产物 (≤1 kDa 的 meretrix lusoria protamex 水解物，MLPH) (150 mg/kg 和 300 mg/kg；口服；一天一次持续 6 周) 对 ob/ob 小鼠具有抗肥胖和抗高血糖作用^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9014-01-1

固体

Off-white to light yellow

碱性蛋白酶

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Riva S, et al. Protease-catalyzed regioselective esterification of sugars and related compounds in anhydrous dimethylformamide[J]. Journal of the American Chemical Society, 1988, 110(2): 584-589.

  [2]. Philipp M, et al. Inhibition of subtilisin by substituted arylboronic acids. FEBS Lett. 1981 Oct 12;133(1):36-8.  [Content Brief]

  [3]. Danieli B, et al. On the regioselectivity of the protease subtilisin towards the acylation of enantiomeric pairs of benzyl and naphthyl glycopyranosides. Part 2[J]. Tetrahedron, 1999, 55(7): 2045-2060.

  [4]. Kim MJ, et al. Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice. Int J Mol Sci. 2022 Apr 5;23(7):4015.  [Content Brief]