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  4. 3β,5α,6β-Trihydroxycholestane

3β,5α,6β-Trihydroxycholestane 

目录号: HY-W010934 纯度: 99.78%
COA 产品使用指南

摩尔计算器

3β,5α,6β-Trihydroxycholestane 是一种天然存在于体内的类固醇，也存在于某些食物中。它属于一类被称为胆甾烷的化合物，与更广为人知的胆固醇密切相关。这种特殊的化合物是由胆固醇在肝脏和其他器官中通过一系列酶促反应形成的。人们对它的潜在健康益处进行了研究，包括减少体内炎症和氧化应激的能力。一些研究表明，它还可能在调节血糖水平和提高胰岛素敏感性方面发挥作用。尽管有这些潜在的好处，但 3β,5α,6β-Trihydroxycholestane 由于其相对较低的含量而未被广泛用作补充剂或药物集中于自然资源和有限的研究。然而，研究人员继续调查其潜在用途和对人类健康的影响。

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3β,5α,6β-Trihydroxycholestane Chemical Structure

3β,5α,6β-Trihydroxycholestane Chemical Structure

CAS No. : 1253-84-5

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规格 价格 是否有货 数量
5 mg ￥900
In-stock
10 mg ￥1500
In-stock
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100 mg   询价  

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Customer Review

Other Forms of 3β,5α,6β-Trihydroxycholestane:

3β,5α,6β-Trihydroxycholestane 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

生物活性

3β,5α,6β-Trihydroxycholestane is a steroid that occurs naturally in the body and is also found in certain foods. It belongs to a class of compounds known as cholestanes, which are closely related to the better known cholesterol. This particular compound is formed from cholesterol through a series of enzymatic reactions in the liver and other organs. It has been studied for its potential health benefits, including its ability to reduce inflammation and oxidative stress in the body. Some research suggests that it may also play a role in regulating blood sugar levels and improving insulin sensitivity. Despite these potential benefits, 3β,5α,6β-Trihydroxycholestane is not widely used as a supplement or medicine due to its relatively low content, focus on natural resources and limited research. However, researchers continue to investigate its potential uses and effects on human health.

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
17.9 μM
Compound: 2
Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
A549 IC50
17.9 μM
Compound: 4
Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
ARPE-19 IC50
19.1 μM
Compound: 2
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
ARPE-19 IC50
19.1 μM
Compound: 4
Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
BJ IC50
18.4 μM
Compound: 2
Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
BJ IC50
18.6 μM
Compound: 4
Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay
Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
HEK293 EC50
1.7 μM
Compound: 8
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
[PMID: 24928400]
HepG2 IC50
10.9 μM
Compound: 2
Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HepG2 IC50
10.9 μM
Compound: 4
Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
HMEC-1 IC50
15.2 μM
Compound: 4
Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay
Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
HT-29 IC50
10.3 μM
Compound: 4
Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay
[PMID: 20931970]
HT-29 IC50
12.9 μM
Compound: 2
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HT-29 IC50
12.9 μM
Compound: 4
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
LAMA-84 IC50
5 μM
Compound: 2
Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
LAMA-84 IC50
5 μM
Compound: 4
Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
MCF7 IC50
13.6 μM
Compound: 2
Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
MCF7 IC50
13.6 μM
Compound: 4
Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
PC-3 IC50
17.4 μM
Compound: 2
Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
PC-3 IC50
17.4 μM
Compound: 4
Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
SH-SY5Y IC50
17.2 μM
Compound: 2
Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
体外研究
(In Vitro)

3β,5α,6β-Trihydroxycholestane 是一种生化试剂，可作为生物材料或有机化合物，用于生命科学相关研究。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

3β,5α,6β-Trihydroxycholestane 相关抗体:
分子量

420.67

Formula

C27H48O3
CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (237.72 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3772 mL 11.8858 mL 23.7716 mL
5 mM 0.4754 mL 2.3772 mL 4.7543 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.94 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.94 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.78%

COA (290 KB) HNMR (270 KB) RP-HPLC (245 KB) MS (74 KB)

产品使用指南 (1538 KB)

3β,5α,6β-Trihydroxycholestane 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3772 mL 11.8858 mL 23.7716 mL 59.4290 mL
5 mM 0.4754 mL 2.3772 mL 4.7543 mL 11.8858 mL
10 mM 0.2377 mL 1.1886 mL 2.3772 mL 5.9429 mL
15 mM 0.1585 mL 0.7924 mL 1.5848 mL 3.9619 mL
20 mM 0.1189 mL 0.5943 mL 1.1886 mL 2.9715 mL
25 mM 0.0951 mL 0.4754 mL 0.9509 mL 2.3772 mL
30 mM 0.0792 mL 0.3962 mL 0.7924 mL 1.9810 mL
40 mM 0.0594 mL 0.2971 mL 0.5943 mL 1.4857 mL
50 mM 0.0475 mL 0.2377 mL 0.4754 mL 1.1886 mL
60 mM 0.0396 mL 0.1981 mL 0.3962 mL 0.9905 mL
80 mM 0.0297 mL 0.1486 mL 0.2971 mL 0.7429 mL
100 mM 0.0238 mL 0.1189 mL 0.2377 mL 0.5943 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3β,5α,6β-Trihydroxycholestane1253-84-5Biochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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