Cyclophosphamide (Synonyms: 环磷酰胺)

目录号: HY-17420 纯度: 99.90%: FAQs
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Cyclophosphamide 是一种合成的 DNA Alkylator，在化学上与氮芥类有关，具有抗肿瘤及免疫抑制活性。

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Cyclophosphamide Chemical Structure

CAS No. : 50-18-0

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200 mg	￥1100	In-stock
500 mg	￥2000	In-stock
1 g	￥3400	In-stock
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Customer Review

Other Forms of Cyclophosphamide:

MCE 顾客使用本产品发表的 29 篇科研文献

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生物活性
纯度 & 产品资料
参考文献

生物活性

Cyclophosphamide is a synthetic alkylating agent chemically related to the nitrogen mustards with antineoplastic activity, a immunosuppressant.

IC₅₀ & Target

DNA Alkylator^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
COS-1	IC₅₀	125.43 μM Compound: CYC-PHO	Cytotoxicity against african green monkey COS1 cells after 24 hrs by MTT assay Cytotoxicity against african green monkey COS1 cells after 24 hrs by MTT assay	[PMID: 23202484]
DU-145	IC₅₀	52.5 μM Compound: CPA	Cytotoxicity against human DU145 cells by MTT assay Cytotoxicity against human DU145 cells by MTT assay	[PMID: 21689869]
HCT-15	IC₅₀	74.32 μM Compound: CYC-PHO	Cytotoxicity against human HCT15 cells after 24 hrs by MTT assay Cytotoxicity against human HCT15 cells after 24 hrs by MTT assay	[PMID: 23202484]
HCT-15	IC₅₀	76.32 μM Compound: CYC-PHO	Growth inhibition of human HCT15 cells after 24 hrs by MTT assay Growth inhibition of human HCT15 cells after 24 hrs by MTT assay	[PMID: 28011220]
HEK-293T	IC₅₀	> 100 μM Compound: CYC-PHO	Growth inhibition of HEK293T cells after 24 hrs by MTT assay Growth inhibition of HEK293T cells after 24 hrs by MTT assay	[PMID: 28011220]
HEK-293T	IC₅₀	> 100 μM Compound: CP	Cytotoxicity against human HEK293T cells by MTT assay Cytotoxicity against human HEK293T cells by MTT assay	[PMID: 20850303]
HeLa	IC₅₀	71.4 μM Compound: CPA	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 21689869]
HEp-2	IC₅₀	> 100 μM Compound: CP	Cytotoxicity against human Hep2 cells by MTT assay Cytotoxicity against human Hep2 cells by MTT assay	[PMID: 20850303]
HepG2	IC₅₀	0.24 μM Compound: CP	Cytotoxicity against Homo sapiens (human) HepG2 cells after 48 hr by MTT assay Cytotoxicity against Homo sapiens (human) HepG2 cells after 48 hr by MTT assay	10.1007/s00044-011-9737-7
HepG2	IC₅₀	52.3 μM Compound: CYC-PHO	Growth inhibition of human HepG2 cells after 24 hrs by MTT assay Growth inhibition of human HepG2 cells after 24 hrs by MTT assay	[PMID: 28011220]
HepG2	IC₅₀	55.3 μM Compound: CYC-PHO	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 23202484]
HL-60	IC₅₀	8.79 μM Compound: CP	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 20850303]
K562	IC₅₀	0.15 μM Compound: CP	Cytotoxicity against Homo sapiens (human) K562 cells after 48 hr by MTT assay Cytotoxicity against Homo sapiens (human) K562 cells after 48 hr by MTT assay	10.1007/s00044-011-9737-7
K562	IC₅₀	0.153 μM Compound: CP	Antiproliferative activity against Homo sapiens (human) K562 cells after 48 hr by MTT assay Antiproliferative activity against Homo sapiens (human) K562 cells after 48 hr by MTT assay	10.1007/s00044-010-9466-3
L1210	IC₅₀	> 300 μM Compound: Cyclophosphamide	Concentration required to reduce the viability of L1210 cells by 50% after 1-h incubation at 37 degrees C Concentration required to reduce the viability of L1210 cells by 50% after 1-h incubation at 37 degrees C	[PMID: 1992116]
MCF7	IC₅₀	0.16 μM Compound: Cyclophosphamide	Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by trypan blue assay Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by trypan blue assay	10.1007/s00044-009-9222-8
MDA-MB-231	IC₅₀	0.09 μM Compound: CP	Cytotoxicity against Homo sapiens (human) MDA-MB-231 cells after 48 hr by MTT assay Cytotoxicity against Homo sapiens (human) MDA-MB-231 cells after 48 hr by MTT assay	10.1007/s00044-011-9737-7
MDA-MB-231	IC₅₀	195.5 μM Compound: Cyclophosphamide	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs	[PMID: 33139111]
NCI-H522	IC₅₀	67.9 μM Compound: CYC-PHO	Cytotoxicity against human NCI-H522 cells after 24 hrs by MTT assay Cytotoxicity against human NCI-H522 cells after 24 hrs by MTT assay	[PMID: 23202484]
NCI-H522	IC₅₀	69.9 μM Compound: CYC-PHO	Growth inhibition of human NCI-H522 cells after 24 hrs by MTT assay Growth inhibition of human NCI-H522 cells after 24 hrs by MTT assay	[PMID: 28011220]
PA-1	IC₅₀	64.12 μM Compound: CYC-PHO	Growth inhibition of human PA1 cells after 24 hrs by MTT assay Growth inhibition of human PA1 cells after 24 hrs by MTT assay	[PMID: 28011220]
PA-1	IC₅₀	64.12 μM Compound: CYC-PHO	Cytotoxicity against human PA1 cells after 24 hrs by MTT assay Cytotoxicity against human PA1 cells after 24 hrs by MTT assay	[PMID: 23202484]
T47D	IC₅₀	69 μM Compound: CYC-PHO	Growth inhibition of human T47D cells after 24 hrs by MTT assay Growth inhibition of human T47D cells after 24 hrs by MTT assay	[PMID: 28011220]
T47D	IC₅₀	70.1 μM Compound: CYC-PHO	Cytotoxicity against human T47D cells after 24 hrs by MTT assay Cytotoxicity against human T47D cells after 24 hrs by MTT assay	[PMID: 23202484]

体外研究
(In Vitro)

Cyclophosphamide 诱导外膜起泡，导致 DNA 片段化，如游离 3'-OH DNA 末端的 TUNEL 染色所示，并诱导 9L/P450 细胞中半胱天冬酶 3 和半胱天冬酶 7 底物 PARP 的裂解。Bcl-2 表达完全阻断用活化 Cyclophosphamide 处理的细胞中启动子半胱天冬酶以及效应子半胱天冬酶 3 的激活。Bcl-2 抑制细胞毒性作用，但不抑制活化 Cyclophosphamide 的细胞生长抑制作用^[1]。
Cyclophosphamide 可逆地抑制 AChE，IC₅₀ 为 511 μM^[2]。
四氯化碳不影响 Cyclophosphamide 或4-羟基 Cyclophosphamide 对培养细胞的直接细胞毒性^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Cyclophosphamide (CP) 可用于构建卵巢早衰模型和骨髓抑制、免疫抑制模型，可通过肝酶 (细胞色素 P450) 酶促转化为细胞毒性 4-羟基环磷酰胺 (4OHCP)。Cyclophosphamide 在动力学方面具有巨大的种间和种内差异。狗微粒体催化 CP 生物活化的效率比人微粒体高 55 倍，比猫微粒体高 2.8 倍，比小鼠微粒体高 1.2 倍。

1. 卵巢早衰模型^[6]

致病原理

环磷酰胺 (Cy) 通过引起原始卵泡激活而诱发卵巢功能不全 (POI)。

具体造模方法：

小鼠：Balb/C • 雌性 • 5 周龄
给药方式：150 mg/kg • 腹腔注射 • 单剂量

Note

造模成功指标

卵巢初级卵泡数量减少。

相关产品： /

拮抗产品： HY-P74413

2. 骨髓抑制和免疫抑制^[7]

致病原理

环磷酰胺通过干扰骨髓 (BM) 细胞的增殖和分化来诱导骨髓抑制。

具体造模方法：

小鼠：Swiss • 雄性 • 6 周龄
给药方式：150 mg/kg • 腹腔注射 • 单剂量^[7].

Note

引起骨髓组织结构的重要变化，降低骨髓/红细胞比率，并减少血液白细胞数量。

造模成功指标

引起骨髓组织结构的重要变化，降低骨髓/红细胞比率，并减少血液白细胞数量。

相关产品： /

拮抗产品： HY-N0045

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six to eight-week old female C3H/HeN mice bearing SW1 tumors^[4]
Dosage:	2 mg/mouse
Administration:	Injected i.p.; 2mg/mouse in 0.1 mL PBS; 4 days
Result:	Increased the percentage of cells that stained for CD3, CD4 or CD8 in both spleens and tumors.

Clinical Trial

分子量

261.09

Formula

C₇H₁₅Cl₂N₂O₂P

CAS 号

50-18-0

性状

固体

颜色

White to off-white

中文名称

环磷酰胺

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

溶解性数据

细胞实验：

DMSO 中的溶解度 : 100 mg/mL (383.01 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : 33.33 mg/mL (127.66 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.8301 mL	19.1506 mL	38.3011 mL
5 mM	0.7660 mL	3.8301 mL	7.6602 mL
10 mM	0.3830 mL	1.9151 mL	3.8301 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.58 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.58 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.58 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 25 mg/mL (95.75 mM); 澄清溶液; 超声助溶 (<60°C)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.90%

选择批次：

Data Sheet (687 KB) SDS (644 KB)

COA (293 KB) HNMR (242 KB) RP-HPLC (235 KB) MS (85 KB) 元素分析报告 (213 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Schwartz PS, et al. Cyclophosphamide induces caspase 9-dependent apoptosis in 9L tumor cells. Mol Pharmacol. 2001 Dec;60(6):1268-1279. [Content Brief]

[2]. al-Jafari AA, et al. Inhibition of human acetylcholinesterase by cyclophosphamide. Toxicology. 1995 Jan 19;96(1):1-6. [Content Brief]

[3]. Harris RN, et al. Carbon tetrachloride-induced increase in the antitumor activity of cyclophosphamide in mice: a pharmacokineticstudy. Cancer Chemother Pharmacol. 1984;12(3):167-72. [Content Brief]

[4]. Liu P, et al. Administration of cyclophosphamide changes the immune profile of tumor-bearing mice. J Immunother. 2010 Jan;33(1):53-9. [Content Brief]

[5]. Dominique A Ramirez, et al. Kinetics of Cyclophosphamide Metabolism in Humans, Dogs, Cats, and Mice and Relationship to Cytotoxic Activity and Pharmacokinetics. Drug Metab Dispos. 2019, 47, 3. [Content Brief]

[6]. H. Roness, et al. Pharmacological administration of recombinant human AMH rescues ovarian reserve and preserves fertility in a mouse model of chemotherapy, without interfering with anti-tumoural effects. J Assist Reprod Genet. 2019, 36, 9.

[7]. Susana Salva, et al. Probiotic Lactobacillus strains protect against myelosuppression and immunosuppression in cyclophosphamide-treated mice. Int Immunopharmacol. 2014, 22, 1. [Content Brief]

Cyclophosphamide 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.8301 mL	19.1506 mL	38.3011 mL	95.7528 mL
	5 mM	0.7660 mL	3.8301 mL	7.6602 mL	19.1506 mL
	10 mM	0.3830 mL	1.9151 mL	3.8301 mL	9.5753 mL
	15 mM	0.2553 mL	1.2767 mL	2.5534 mL	6.3835 mL
	20 mM	0.1915 mL	0.9575 mL	1.9151 mL	4.7876 mL
	25 mM	0.1532 mL	0.7660 mL	1.5320 mL	3.8301 mL
	30 mM	0.1277 mL	0.6384 mL	1.2767 mL	3.1918 mL
	40 mM	0.0958 mL	0.4788 mL	0.9575 mL	2.3938 mL
	50 mM	0.0766 mL	0.3830 mL	0.7660 mL	1.9151 mL
	60 mM	0.0638 mL	0.3192 mL	0.6384 mL	1.5959 mL
	80 mM	0.0479 mL	0.2394 mL	0.4788 mL	1.1969 mL
	100 mM	0.0383 mL	0.1915 mL	0.3830 mL	0.9575 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyclophosphamide50-18-0环磷酰胺DNA Alkylator/CrosslinkerInhibitorinhibitorinhibit

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Cyclophosphamide (Synonyms: 环磷酰胺)

Customer Review

Other Forms of Cyclophosphamide:

MCE 顾客使用本产品发表的 29 篇科研文献

Cyclophosphamide 相关产品

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•同靶点产品:

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Cyclophosphamide (Synonyms: 环磷酰胺)

Customer Review

Other Forms of Cyclophosphamide:

Cyclophosphamide 相关抗体

MCE 顾客使用本产品发表的 29 篇科研文献

Cyclophosphamide 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

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参考文献

Cyclophosphamide 相关抗体:

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Cyclophosphamide 相关分类

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