2. GPCR/G Protein Immunology/Inflammation Anti-infection
  3. CCR HIV
  4. DAPTA

DAPTA  (Synonyms: D-Ala-peptide T-amide; Adaptavir)

目录号: HY-P1034 纯度: 99.85%
COA 产品使用指南

摩尔计算器

DAPTA 是一种合成肽，为 CCR5 抑制剂，同时具有抗 HIV 的活性。

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Custom Peptide Synthesis

DAPTA Chemical Structure

DAPTA Chemical Structure

CAS No. : 106362-34-9

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥600
In-stock
5 mg ￥1100
In-stock
10 mg ￥1900
In-stock
25 mg ￥3600
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

DAPTA 相关抗体

Top Publications Citing Use of Products

查看 CCR 亚型特异性产品:

查看所有亚型
CCR1 CCR2 CCR3 CCR4 CCR5 CCR6 CCR7 CCR8 CCR9

查看 HIV 亚型特异性产品:

查看所有亚型
HIV HIV-1 HIV-2

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

DAPTA is a synthetic peptide, functions as a viral entry inhibitor by targeting selectively CCR5, and shows potent anti-HIV activities.

IC50 & Target[2]

HIV-1

 

gp120BaL/sCD4-CCR5

55 pM (IC50, in Cf2Th/synR5 cells)

gp120CMCM235/sCD4-CCR5

0.32 nM (IC50, in Cf2Th/synR5 cells)

体外研究
(In Vitro)

DAPTA (1 nM) inhibits HIV-1 replication in monocytes/macrophages (M/M) by >90%. DAPTA blocks HIV entry and prevents HIV-1 infection. DAPTA reduces CCR5 mAb binding in human primary macrophages. DAPTA potently blocks R5 gp120-mediated neuronal apoptosis. DAPTA is even more potent in preventing neuronal apoptosis than the CCR5 antagonist TAK-779[1]. DAPTA potently inhibits specific CD4-dependent binding of gp120 Bal (IC50 = 0.06 nM) and CM235 (IC50 = 0.32 nM) to CCR5. DAPTA (1 nM) blocks formation of the gp120/sCD4 complex with CCR5. DAPTA inhibits the binding of gp120BaL/sCD4 to CCR5 (Cf2Th/synR5) cells with IC50 of 55 ± 0.08 pM[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

DAPTA 相关抗体:
分子量

856.88

Formula

C35H56N10O15
CAS 号
性状

固体

颜色

White to off-white

Sequence

Ala-Ser-Thr-Thr-Thr-Asn-Tyr-Thr-NH2
Sequence Shortening

ASTTTNYT-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验： 

H2O 中的溶解度 : 50 mg/mL (58.35 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1670 mL 5.8351 mL 11.6702 mL
5 mM 0.2334 mL 1.1670 mL 2.3340 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： PBS

    Solubility: 10 mg/mL (11.67 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料
参考文献
Kinase Assay
[2]

A novel FITC-labeled tracer from soluble gp120 proteins (25 g/mL) is prepared using a Fluorescent protein labeling kit, according to the manufacture's instructions. Uncoupled FLUOS is removed by Sephadex G-10 column filtration. The molar ratio between FLUOS-labeling molecules and protein is from 3.5 to 4.5 fluorescence molecules per molecule of gp120. The concentration of fluorescent-labeled proteins is measured by Bradford assay and Western blotting by using calibrating amounts of soluble molecules with known concentration. Binding assays are performed in binding buffer, in final volume 100l. Binding is carried out for 1 h at 37°C in 96-well filter plates. Unbound-labeled proteins are removed by rapid vacuum filtration and ishing using a 96-well plates manifold. Each binding mix is washed five times with 0.2 mL (total volume of 1.0 mL/well) cold ishing buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM CaCl2). Filters are counted with a fluorescent plate reader at 495/530 nm.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

DAPTA 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.1670 mL 5.8351 mL 11.6702 mL 29.1756 mL
5 mM 0.2334 mL 1.1670 mL 2.3340 mL 5.8351 mL
10 mM 0.1167 mL 0.5835 mL 1.1670 mL 2.9176 mL
15 mM 0.0778 mL 0.3890 mL 0.7780 mL 1.9450 mL
20 mM 0.0584 mL 0.2918 mL 0.5835 mL 1.4588 mL
25 mM 0.0467 mL 0.2334 mL 0.4668 mL 1.1670 mL
30 mM 0.0389 mL 0.1945 mL 0.3890 mL 0.9725 mL
40 mM 0.0292 mL 0.1459 mL 0.2918 mL 0.7294 mL
50 mM 0.0233 mL 0.1167 mL 0.2334 mL 0.5835 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DAPTA106362-34-9D-Ala-peptide T-amide AdaptavirCCRHIVCC chemokine receptorHuman immunodeficiency virusInhibitorinhibitorinhibit

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目录号：
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