2. Immunology/Inflammation
  3. PD-1/PD-L1
  4. Pembrolizumab

Pembrolizumab  (Synonyms: 帕博利珠单抗; MK-3475; Lambrolizumab)

目录号: HY-P9902 纯度: 99.17%
COA

摩尔计算器

Pembrolizumab (MK-3475) 是一种人源化 IgG4 抗体，为 PD-1 的抑制剂，用于癌症免疫的研究。

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CAS No. : 1374853-91-4

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5 mg ￥5900
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10 mg ￥7600
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Customer Review

Pembrolizumab 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Pembrolizumab (MK-3475) is a humanized IgG4 antibody inhibiting the programmed cell death 1 (PD-1) receptor, used in cancer immunotherapy.

同型

Human IgG4 kappa
推荐同型对照抗体
种属

Humanized
IC50 & Target

IC50: 0.6 nM (PD-1)
体外研究
(In Vitro)

Pembrolizumab (MK-3475) 可增加细胞因子 IFN-γ、TNF-α 的分泌，增加 A549 细胞的凋亡[2]。
Pembrolizumab 可增强 αROR1-CAR T 介导的细胞毒性，并通过阻断 αROR1-CAR T 细胞中 PD-1::PD-L1 的相互作用，降低共培养的 αROR1-CAR T 和 A549 细胞中的肿瘤形成[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Pembrolizumab 相关抗体:

Cell Cytotoxicity Assay[2]

Cell Line: αROR1-CAR T and A549 cells
Concentration: 10 μg/mL
Incubation Time: Adding Pembrolizumab after A549-Red-Fluc cells preconditioned in elevated pressure (+ 100 mmHg) for 7 days.
Result: Enhanced αROR1-CAR T killing of A549 lung cancer cells under elevated pressure.

Apoptosis Analysis[2]

Cell Line: A549 cells
Concentration: 10 μg/mL
Incubation Time:
Result: Increased the apoptotic cell death in A549 cells, and improve the αROR1-CAR T-mediated cytotoxicity.
体内研究
(In Vivo)

Pembrolizumab 可抑制经压力预处理的 A549 异种移植小鼠的肿瘤大小[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-week-old male BALB/c athymic nude mice[2]
Dosage: 5 mg/kg
Administration: Pembrolizumab (5 mg/kg; Intravenous injection; Once)
Result: Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.
Clinical Trial
CAS 号
性状

液体

颜色

Colorless to light yellow

中文名称

帕博利珠单抗； 派姆单抗
运输条件

Shipping with dry ice.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Format
  • Human IgG4 kappa
Biological Activity
  • Flow cytometry analysis of PD-1 overexpression HEK293 cells labelling PD-1 with Pembrolizumab at 67nM (Red). Goat anti human IgG Fc(Alexa Fluor488) was used as the secondary antibody at 1/400 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells (Gray).
  • Loaded PD-1 Protein, Human (143a.a, HEK293, His, HY-P7396) on AHC2 biosensor, can bind Pembrolizumab with an affinity constant of 1.898E-09 M as determined in BLI assay.
  • Immobilized PD-1 Protein, Human can bind Pembrolizumab. The EC50 for this effect is 1.8 ng/mL.
纯度 & 产品资料

纯度: 99.17%

COA (264 KB) SDS-PAGE (86 KB) SEC-HPLC (90 KB)

抗体抑制剂使用指南 (1152 KB)
参考文献

Pembrolizumab 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pembrolizumab1374853-91-4MK-3475 Lambrolizumab帕博利珠单抗 派姆单抗MK3475MK 3475PD-1/PD-L1PD-1/Programmed death-ligand 1PD-1 inhibitorIgG4 antibody cancerimmunologyInhibitorinhibitorinhibit

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