Protirelin (Synonyms: 普罗瑞林; Thyrotropin-releasing-hormone; TRH)

目录号: HY-P0002 纯度: 99.51%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Protirelin 是一种高度保守的神经肽，发挥促甲状腺激素 (TSH) 水平的激素控制作用，及神经调节功能。

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Custom Peptide Synthesis

Protirelin Chemical Structure

CAS No. : 24305-27-9

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规格	价格	是否有货
5 mg	￥600	In-stock
10 mg	￥1000	In-stock
25 mg	￥1700	In-stock
50 mg	￥2700	In-stock
100 mg		询价
200 mg		询价

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Customer Review

Other Forms of Protirelin:

Thyrotropin-Releasing Hormone (TRH), Free Acid In-stock
Protirelin acetate In-stock

MCE 顾客使用本产品发表的 3 篇科研文献

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Protirelin is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

IC₅₀ & Target

Thyroid hormone receptor

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	0.0005 μM Compound: TRH	Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay	[PMID: 26854379]
HEK293	EC₅₀	0.00052 μM Compound: TRH	Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as induction of changes in intracellular Ca2+ level by FLIPR method Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as induction of changes in intracellular Ca2+ level by FLIPR method	[PMID: 26216015]
HEK293	EC₅₀	0.001 μM Compound: TRH	Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as Ca2+ release by FLIPR assay	[PMID: 26854379]
HEK293	EC₅₀	0.001 μM Compound: TRH	Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as induction of changes in intracellular Ca2+ level by FLIPR method Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as induction of changes in intracellular Ca2+ level by FLIPR method	[PMID: 26216015]
HEK293	IC₅₀	0.0026 μM Compound: TRH	Displacement of [3H]-1-MeHis-TRH from mouse TRH-R1 expressed in HEK293 cells incubated up to 4 hrs by scintillation counting method Displacement of [3H]-1-MeHis-TRH from mouse TRH-R1 expressed in HEK293 cells incubated up to 4 hrs by scintillation counting method	[PMID: 26216015]
HEK293	EC₅₀	0.003 μM Compound: TRH	Signaling (activation) potency for thyrotropin releasing hormone receptor 2 expressed in HEK 293EM cells with CREB-luciferase reporter upon incubation with the compound for 6 hr at 37 degree C and pH 7.8 Signaling (activation) potency for thyrotropin releasing hormone receptor 2 expressed in HEK 293EM cells with CREB-luciferase reporter upon incubation with the compound for 6 hr at 37 degree C and pH 7.8	[PMID: 16162016]
HEK293	EC₅₀	0.003 μM Compound: TRH	Signaling (activation) potency for thyrotropin releasing hormone receptor 1 expressed in HEK 293EM cells with CREB-luciferase reporter upon incubation with the compound for 6 hr at 37 degree C and pH 7.8; Range = 0.002-0.004 uM Signaling (activation) potency for thyrotropin releasing hormone receptor 1 expressed in HEK 293EM cells with CREB-luciferase reporter upon incubation with the compound for 6 hr at 37 degree C and pH 7.8; Range = 0.002-0.004 uM	[PMID: 16162016]
HEK293	EC₅₀	0.0031 μM Compound: TRH	Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as IP1 formation after 1 hr by ELISA Agonist activity at mouse TRH-R2 expressed in HEK293 cells assessed as IP1 formation after 1 hr by ELISA	[PMID: 26854379]
HEK293	EC₅₀	0.0032 μM Compound: TRH	Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as IP1 formation after 1 hr by ELISA Agonist activity at mouse TRH-R1 expressed in HEK293 cells assessed as IP1 formation after 1 hr by ELISA	[PMID: 26854379]
HEK293	IC₅₀	0.007 μM Compound: TRH	Displacement of [3H]-1-MeHis-TRH from mouse TRH-R2 expressed in HEK293 cells incubated up to 4 hrs by scintillation counting method Displacement of [3H]-1-MeHis-TRH from mouse TRH-R2 expressed in HEK293 cells incubated up to 4 hrs by scintillation counting method	[PMID: 26216015]
HEK293	IC₅₀	0.007 μM Compound: TRH	Displacement of [3H]-Me-His-TRH from mouse TRH-R2 expressed in HEK293 cells preincubated for 15 mins measured after 4 hrs Displacement of [3H]-Me-His-TRH from mouse TRH-R2 expressed in HEK293 cells preincubated for 15 mins measured after 4 hrs	[PMID: 26854379]
HEK293	IC₅₀	0.026 μM Compound: TRH	Displacement of [3H]-Me-His-TRH from mouse TRH-R1 expressed in HEK293 cells preincubated for 15 mins measured after 4 hrs Displacement of [3H]-Me-His-TRH from mouse TRH-R1 expressed in HEK293 cells preincubated for 15 mins measured after 4 hrs	[PMID: 26854379]

体内研究
(In Vivo)

Protirelin (TRH) is an evolutionarily ancient neuropeptide, having its origin before the divergence of protostomes and deuterostomes, and may ancestrally have been involved in the control of postembryonic growth and reproduction^[1]. The effect of the thyrotropin-releasing hormone (Protirelin, TRH), one of the hypothalamic releasing hormones, on body temperature is investigated in the rat. Protirelin, in doses of 1, 5, 10 and 20 mg/kg, is injected intraperitoneally to male Wistar rats weighing 200-250 g. Protirelin causes a temporary rise in body temperature dose-dependently. The thyroidectomized rats injected 20 mg/kg of Protirelin which induces a significant hyperthermia in the sham-operated animals, fail to show a rise in body temperature. The present results suggest that a release of thyroid hormone might participate in the hyperthermic action of Protirelin^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

362.38

Formula

C₁₆H₂₂N₆O₄

CAS 号

24305-27-9

性状

固体

颜色

White to off-white

Sequence

{pGlu}-His-Pro-NH2

Sequence Shortening

{pGlu}-HP-NH2

中文名称

普罗瑞林

结构分类

Others

初始来源

内源性代谢物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 250 mg/mL (689.88 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.7595 mL	13.7977 mL	27.5953 mL
5 mM	0.5519 mL	2.7595 mL	5.5191 mL
10 mM	0.2760 mL	1.3798 mL	2.7595 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: 99.51%

选择批次：

Data Sheet (609 KB) SDS (252 KB)

COA (291 KB) HNMR (245 KB) LCMS (88 KB) 元素分析报告 (215 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief]

[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Animal Administration ^[2]	Rats^[2] Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief] [2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Animal Administration
^[2]

Rats^[2]
Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief]

[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Protirelin 相关分类

Inflammation/Immunology Endocrinology

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7595 mL	13.7977 mL	27.5953 mL	68.9884 mL
	5 mM	0.5519 mL	2.7595 mL	5.5191 mL	13.7977 mL
	10 mM	0.2760 mL	1.3798 mL	2.7595 mL	6.8988 mL
	15 mM	0.1840 mL	0.9198 mL	1.8397 mL	4.5992 mL
	20 mM	0.1380 mL	0.6899 mL	1.3798 mL	3.4494 mL
	25 mM	0.1104 mL	0.5519 mL	1.1038 mL	2.7595 mL
	30 mM	0.0920 mL	0.4599 mL	0.9198 mL	2.2996 mL
	40 mM	0.0690 mL	0.3449 mL	0.6899 mL	1.7247 mL
	50 mM	0.0552 mL	0.2760 mL	0.5519 mL	1.3798 mL
	60 mM	0.0460 mL	0.2300 mL	0.4599 mL	1.1498 mL
	80 mM	0.0345 mL	0.1725 mL	0.3449 mL	0.8624 mL
	100 mM	0.0276 mL	0.1380 mL	0.2760 mL	0.6899 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Protirelin24305-27-9Thyrotropin-releasing-hormone TRH普罗瑞林Thyroid Hormone ReceptorEndogenous MetaboliteTHRInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Protirelin (Synonyms: 普罗瑞林; Thyrotropin-releasing-hormone; TRH)

Customer Review

Other Forms of Protirelin:

MCE 顾客使用本产品发表的 3 篇科研文献