Protirelin acetate (Synonyms: 醋酸普罗瑞林; Thyrotropin-releasing-hormone acetate; TRH acetate)

目录号: HY-P0002A 纯度: 99.93%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Protirelin Acetate 是一种高度保守的神经肽，发挥促甲状腺激素 (TSH) 水平的激素控制作用，及神经调节功能。

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Custom Peptide Synthesis

Protirelin acetate Chemical Structure

CAS No. : 120876-23-5

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规格	价格	是否有货
10 mM * 1 mL in Water	￥462	In-stock
5 mg	￥420	In-stock
10 mg	￥700	In-stock
50 mg	￥1900	In-stock
100 mg	￥2800	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

Other Forms of Protirelin acetate:

Thyrotropin-Releasing Hormone (TRH), Free Acid In-stock
Protirelin In-stock

MCE 顾客使用本产品发表的 3 篇科研文献

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Protirelin Acetate is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions.

IC₅₀ & Target

Thyroid hormone receptor

体内研究
(In Vivo)

Protirelin (TRH) 是一种进化上古老的神经肽，其起源早于原口动物和后口动物的分化，并且可能在祖先上参与了胚后生长和繁殖的控制^[1]。在大鼠身上研究了促甲状腺素释放激素 (Protirelin，TRH)? (一种下丘脑释放激素) 对体温的影响。将剂量为 1、5、10 和 20 mg/kg 的 Protirelin 腹膜内注射到体重 200-250 g 的雄性 Wistar 大鼠。Protirelin 剂量依赖性地导致体温暂时升高。甲状腺切除的大鼠注射 20 mg/kg 的 Protirelin，这会在假手术动物中引起显著的体温过高，但未能显示出体温升高。目前的结果表明，甲状腺激素的释放可能参与了 Protirelin 的温热作用^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

452.46

Formula

C₁₆H₂₂N₆O₄.3/2C₂H₄O₂

CAS 号

120876-23-5

性状

固体

颜色

White to off-white

Sequence

{pGlu}-His-Pro-NH2

Sequence Shortening

{pGlu}-HP-NH2

中文名称

醋酸普罗瑞林

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 233.33 mg/mL (515.69 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : 50 mg/mL (110.51 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.2101 mL	11.0507 mL	22.1014 mL
5 mM	0.4420 mL	2.2101 mL	4.4203 mL
10 mM	0.2210 mL	1.1051 mL	2.2101 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.60 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.60 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.60 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (221.01 mM); 澄清溶液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.98%

选择批次：

Data Sheet (648 KB) SDS (252 KB)

COA (180 KB) HNMR (258 KB) RP-HPLC (223 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief]

[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Animal Administration ^[2]	Rats^[2] Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief] [2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Animal Administration
^[2]

Rats^[2]
Ninety male Wistar rats weighing 200-250 g are subjected to the study. In the first experiment, 50 rats are divided into five groups randomly. Four doses of Protirelin dissolved in physiological saline, i. e., 1 mg/kg, 5mg/kg, 10 mg/kg and 20 mg/kg, are administered intraperitoneally to the four groups and saline to the remaining control group. Rectal temperature is measured at the place 5 cm inner from the anus with the electronic thermister before and after treatment with Protirelin or saline. In the second experiment, 40 rats are thyroidectomized or sham-operated under the anesthesia by thiopental sodium. Ten days after the operation, 20 mg/kg of Protirelin or saline is administered to the thyroidectomized and sham-operated animals by i. p. and rectal temperature is measured using the same method as used in the first experiment. These two experiments are undertaken from 1 p, m, to 4 p. m. and the room temperature is kept at 24±1°C through the experiments including the breeding period. For a statistical analysis, Student's t test (two-tailed) is adopted.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Van Sinay E, et al. Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4065-E4074. [Content Brief]

[2]. Noda Y, et al. Hyperthermia induced by thyrotropin-releasing hormone (TRH, Protirelin) in the rat. Kurume Med J. 1979;26(2):107-12. [Content Brief]

Protirelin acetate 相关分类

Inflammation/Immunology Endocrinology

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.2101 mL	11.0507 mL	22.1014 mL	55.2535 mL
	5 mM	0.4420 mL	2.2101 mL	4.4203 mL	11.0507 mL
	10 mM	0.2210 mL	1.1051 mL	2.2101 mL	5.5254 mL
	15 mM	0.1473 mL	0.7367 mL	1.4734 mL	3.6836 mL
	20 mM	0.1105 mL	0.5525 mL	1.1051 mL	2.7627 mL
	25 mM	0.0884 mL	0.4420 mL	0.8841 mL	2.2101 mL
	30 mM	0.0737 mL	0.3684 mL	0.7367 mL	1.8418 mL
	40 mM	0.0553 mL	0.2763 mL	0.5525 mL	1.3813 mL
	50 mM	0.0442 mL	0.2210 mL	0.4420 mL	1.1051 mL
	60 mM	0.0368 mL	0.1842 mL	0.3684 mL	0.9209 mL
	80 mM	0.0276 mL	0.1381 mL	0.2763 mL	0.6907 mL
	100 mM	0.0221 mL	0.1105 mL	0.2210 mL	0.5525 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Protirelin120876-23-5Thyrotropin-releasing-hormoneTRH醋酸普罗瑞林Thyroid Hormone ReceptorTHRInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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Protirelin acetate (Synonyms: 醋酸普罗瑞林; Thyrotropin-releasing-hormone acetate; TRH acetate)

Customer Review

Other Forms of Protirelin acetate:

MCE 顾客使用本产品发表的 3 篇科研文献