2. MAPK/ERK Pathway PI3K/Akt/mTOR Apoptosis Autophagy Metabolic Enzyme/Protease Immunology/Inflammation NF-κB
  3. JNK Akt Apoptosis Autophagy Reactive Oxygen Species
  4. Actein

Actein  (Synonyms: 黄肉楠碱)

目录号: HY-N6872 纯度: 99.25%
COA 产品使用指南

摩尔计算器

Actein 是从 Cimicifuga foetida 的根茎中分离的三萜糖苷，具有抑制癌细胞的效果。Actein 通过促进 ROS/JNK 活化和钝化膀胱癌中的 AKT 途径来抑制细胞增殖，诱导自噬 (autophagy) 和凋亡 (apoptosis)。Actein 在体内几乎没有毒性。

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Actein Chemical Structure

Actein Chemical Structure

CAS No. : 18642-44-9

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1 mg ￥2050
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5 mg ￥4500
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Customer Review

Other Forms of Actein:

Actein 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Actein, a triterpene glycoside, shows an inhibitory effect on cancer cells, which is isolated from the rhizomes of Cimicifuga foetida. Actein suppresses cell proliferation, induces autophagy and apoptosis through promoting ROS/JNK activation, and blunting AKT pathway in bladder cancer. Actein has little toxicity in vivo[1][2][3].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
HCC1806 IC50
> 20 μM
Compound: 1
Antiproliferative activity against human HCC1806 cells assessed as inhibition of cell growth incubated for 36 hrs by SRB assay
Antiproliferative activity against human HCC1806 cells assessed as inhibition of cell growth incubated for 36 hrs by SRB assay
[PMID: 37121522]
HeLa IC50
> 10 μM
Compound: 11
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
[PMID: 25136911]
HL-60 IC50
> 10 μM
Compound: 11
Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
Cytotoxicity against human HL60 cells after 48 hrs by MTT assay
[PMID: 25136911]
MCF7 IC50
> 10 μM
Compound: 11
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
[PMID: 25136911]
MCF7 IC50
14 μM
Compound: 1
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 96 hrs by coulter counter analysis
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 96 hrs by coulter counter analysis
[PMID: 37121522]
MDA-MB-231 IC50
> 20 μM
Compound: 1
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 36 hrs by SRB assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 36 hrs by SRB assay
[PMID: 37121522]
MDA-MB-231 IC50
74 μM
Compound: 1
Antiproliferative activity against human MDA-MB-231 cells overexpressing ERnegative and Her2 assessed as inhibition of cell growth incubated for 96 hrs by coulter counter analysis
Antiproliferative activity against human MDA-MB-231 cells overexpressing ERnegative and Her2 assessed as inhibition of cell growth incubated for 96 hrs by coulter counter analysis
[PMID: 37121522]
NCI-H460 IC50
> 10 μM
Compound: 11
Cytotoxicity against human H460 cells after 48 hrs by MTT assay
Cytotoxicity against human H460 cells after 48 hrs by MTT assay
[PMID: 25136911]
Oocyte EC50
36 μM
Compound: 1
Allosteric modulation of alpha1beta2gamma2S GABAA receptor expressed in Xenopus laevis oocytes assessed as stimulation of GABA-induced chloride current by electrophysiology
Allosteric modulation of alpha1beta2gamma2S GABAA receptor expressed in Xenopus laevis oocytes assessed as stimulation of GABA-induced chloride current by electrophysiology
[PMID: 21082802]
SMMC-7721 IC50
> 10 μM
Compound: 11
Cytotoxicity against human SMMC-7721 cells after 48 hrs by MTT assay
Cytotoxicity against human SMMC-7721 cells after 48 hrs by MTT assay
[PMID: 25136911]
体外研究
(In Vitro)

Actein (0-30 µM, 24 小时) 联合肿瘤坏死因子相关凋亡诱导配体 (TRAIL) 显著抑制胃癌细胞的增殖，并通过 Caspase-3 和 p53 信号通路诱导细胞凋亡 (apoptosis)[1]。
Actein (0-40 μM，24 小时) 能够抑制膀胱癌细胞的增殖，诱导 G2/M 细胞周期停滞，自噬 (autophagy) 和凋亡(apoptosis)[2]。
Actein (0-20 μM，24 小时) 增强了 BIU-87 和 T24 细胞中的活性氧 (ROS) 生成和 JNK 磷酸化，同时抑制了 AKT 通路[2]。
Actein (10 nM 和 10 μM, 5-10 分钟) 增加了乳腺癌细胞和结肠癌细胞胞质中的钙释放[3]。
Actein (0-40 μg/mL，48 小时) 通过降低 293T (null) 和 293T (NF-κB) 细胞中 IKKβ 蛋白的水平，抑制 NF-κB 和 MEK 通路[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Actein 相关抗体:

Cell Viability Assay[1]

Cell Line: SNU-216 and AGS gastric cancer cell lines
Concentration: 0-30 µM
Incubation Time: 24 h
Result: Exhibited a strong inhibitory effect on gastric cancer cell proliferation in a dose-dependent manner combined with TRAIL in gastric cancer cells.

Immunofluorescence[2]

Cell Line: BIU-87 and T24 cells
Concentration: 0-20 μM
Incubation Time: 24 h
Result: Exhibited dose-increased levels of oxidation-sensitive red fluorescence, indicating the elevation of ROS in BIU-87 and T24 cells.

Western Blot Analysis[1]

Cell Line: SNU-216 and AGS gastric cancer cell lines
Concentration: 0-30 µM
Incubation Time: 24 h
Result: Dose-dependently reduced the AKT/mTOR and JAK2/STAT3 activity, while p38 and JNK were highly phosphorylated in BIU-87 and T24 cells.

Western Blot Analysis[3]

Cell Line: 293T (null) and 293T (NF-κB) cells
Concentration: 0-40 μg/mL
Incubation Time: 48 h
Result: Decreased the level of IKKβ protein in a dose dependent manner in 293T (null) and 293T (NF-κB) cells.
分子量

676.83

Formula

C37H56O11
CAS 号
性状

固体

颜色

White to off-white

中文名称

黄肉楠碱
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (147.75 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4775 mL 7.3874 mL 14.7748 mL
5 mM 0.2955 mL 1.4775 mL 2.9550 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.25%

COA (291 KB) RP-HPLC (213 KB)

产品使用指南 (1538 KB)
参考文献

Actein 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4775 mL 7.3874 mL 14.7748 mL 36.9369 mL
5 mM 0.2955 mL 1.4775 mL 2.9550 mL 7.3874 mL
10 mM 0.1477 mL 0.7387 mL 1.4775 mL 3.6937 mL
15 mM 0.0985 mL 0.4925 mL 0.9850 mL 2.4625 mL
20 mM 0.0739 mL 0.3694 mL 0.7387 mL 1.8468 mL
25 mM 0.0591 mL 0.2955 mL 0.5910 mL 1.4775 mL
30 mM 0.0492 mL 0.2462 mL 0.4925 mL 1.2312 mL
40 mM 0.0369 mL 0.1847 mL 0.3694 mL 0.9234 mL
50 mM 0.0295 mL 0.1477 mL 0.2955 mL 0.7387 mL
60 mM 0.0246 mL 0.1231 mL 0.2462 mL 0.6156 mL
80 mM 0.0185 mL 0.0923 mL 0.1847 mL 0.4617 mL
100 mM 0.0148 mL 0.0739 mL 0.1477 mL 0.3694 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Actein18642-44-9黄肉楠碱JNKAktApoptosisAutophagyReactive Oxygen Speciesc-Jun N-terminal kinasePKBProtein kinase Btriterpene glycosidecancer cellscell proliferationautophagyapoptosisROS/JNK activationAKT pathwayInhibitorinhibitorinhibit

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