Dextran sulfate sodium salt (MW 5000)  (Synonyms: 硫酸葡聚糖钠盐 (MW 5000); DSS (MW 5000); DXS (MW 5000))

Dextran sulfate sodium salt (MW 5000) is a polymer of anhydroglucose with a molecular weight of 5000. Dextran sulfate sodium salt (MW 5000) can be used to induce acute colitis and cause apoptosis of colonic epithelial cells in mice. The concentration dose used in the study was 5% (in feed, w/w). The sulfated polysaccharide dextran sulfate is also an effective inhibitor of HIV. Dextran sulfate sodium salt (MW 5000) can significantly inhibit HIV-1 replication at a concentration that does not significantly inhibit the blood coagulation process. Dextran sulfate sodium salt (MW 5000) protects MT-4 cells from HIV-1-induced cellular pathogenicity. Dextran sulfate-induced colitis can be inhibited by Puerarin (HY-N0145), Baicalein (HY-N0196), β-Caryophyllene (HY-N1415).

Dextran sulfate sodium salt (MW 5000)（25 μg/mL；24 h）可完全保护 MT-4 细胞免受 HIV-1 诱导的细胞致病性^[1]。
Dextran sulfate sodium salt (DSS) 被 2-氨基吡啶标记后 (得到 pyridylamino-DSS 或 PA-DSS)，用于检测其在 Caco-2 细胞中的代谢情况。PA-DSS (MW 5000) (3%；24 h) 可快速抑制 Caco-2 细胞的能量代谢^[2]。
用乙醇固定细胞，用丙酮渗透，用 0.05% 甲苯胺蓝溶液染色 20 分钟。结果显示^[2]：
Dextran sulfate sodium salt (MW 5000) 在细胞内发生异色反应，显色结果说明其主要与细胞核结合，并在进入细胞后解聚到 MW 2500 以下。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Dextran sulfate sodium salt (MW 5000) 主要在结肠而不是胃或者小肠中诱导炎症。Dextran sulfate sodium salt (MW 5000) (3%，混入饲料；喂食 8 天) 可诱发小鼠的结肠炎，引起小鼠体重减轻，黏膜损伤，以及降低细胞有丝分裂率和诱导结肠上皮细胞凋亡。Dextran sulfate sodium salt (MW 5000) 效果比 Dextran sulfate sodium salt (MW 2500) 更好 (在同时诱导后的第6天，各项指标出现统计学差异)^[2]。
Dextran sulfate sodium salt (平均 MW 5000) (3%，加入饮用水；灌胃 10 天) 可诱发大鼠的结肠炎，引起大鼠便血，RBC 和 WBC 指标降低^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

5000(Average)

9011-18-1

固体

Off-white to light yellow

硫酸葡聚糖；硫酸葡聚糖钠；葡聚糖硫酸酯钠；葡聚糖硫酸氢钠；糖酐酯

Room temperature in continental US; may vary elsewhere.

Store at room temperature, keep dry and cool

• [1]. Baba M, et al. Mechanism of inhibitory effect of dextran sulfate and heparin on replication of human immunodeficiency virus in vitro. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6132-6.  [Content Brief]

  [2]. Araki Y, et, al. Dextran sulfate sodium administered orally is depolymerized in the stomach and induces cell cycle arrest plus apoptosis in the colon in early mouse colitis. Oncol Rep. 2012 Nov;28(5):1597-605.  [Content Brief]

  [3]. Hori Y, et al. Effects of chondroitin sulfate on colitis induced by dextran sulfate sodium in rats. Jpn J Pharmacol. 2001 Feb;85(2):155-60.  [Content Brief]

  [4]. Martin JC, et al. Dextran Sulfate Sodium (DSS)-Induced Acute Colitis in the Rat. Methods Mol Biol. 2016;1371:197-203. [Content Brief]  [Content Brief]

  [5]. Jeon YD, et al. Puerarin inhibits inflammation and oxidative stress in dextran sulfate sodium-induced colitis mice model. Biomed Pharmacother. 2020 Apr;124:109847.  [Content Brief]

  [6]. Zhong X, et al. Baicalein Inhibits Dextran Sulfate Sodium-induced Mouse Colitis. J Cancer Prev. 2019 Jun;24(2):129-138.  [Content Brief]

  [7]. Bento AF, et al. β-Caryophyllene inhibits dextran sulfate sodium-induced colitis in mice through CB2 receptor activation and PPARγ pathway. Am J Pathol. 2011 Mar;178(3):1153-66.  [Content Brief]