2. Immunology/Inflammation
  3. COX NO Synthase
  4. iNOS/COX-2-IN-1

iNOS/COX-2-IN-1 (Compound 12e) 是 cyclooxygenase-2 (COX-2) 和 inducible nitric oxide synthase (iNOS) 的抑制剂。iNOS/COX-2-IN-1 抑制了 NF-κB 和 MAPKs 信号通路，从而发挥抗炎作用。

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iNOS/COX-2-IN-1 Chemical Structure

iNOS/COX-2-IN-1 Chemical Structure

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Customer Review

iNOS/COX-2-IN-1 相关抗体

Top Publications Citing Use of Products

查看 COX 亚型特异性产品:

查看所有亚型
COX COX-1 COX-2 COX-3

查看 NO Synthase 亚型特异性产品:

查看所有亚型
nNOS iNOS eNOS

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

iNOS/COX-2-IN-1 (Compound 12e) is an inhibitor of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). iNOS/COX-2-IN-1 inhibits the NF-κB and MAPKs signaling pathways and thus exerts anti-inflammatory effects[1].

IC50 & Target

COX-2

 

iNOS

 

体外研究
(In Vitro)

iNOS/COX-2-IN-1 (0.625-40 μM; 24 h) 在 5 μM 浓度下对 RAW 264.7 细胞无明显细胞毒性[1]。
iNOS/COX-2-IN-1 (2.5-10 μM; 1 h) 显著抑制了 LPS 诱导的 NO 生成，NO 抑制率为 45.38 % (5 μM)。浓度依赖性地抑制了 TNF-α 和 COX-2 的表达。效抑制促炎性细胞因子的 mRNA 表达。通过抑制 NF-κB 信号通路的激活，发挥其抗炎作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

iNOS/COX-2-IN-1 相关抗体:

Cell Cytotoxicity Assay[1]

Cell Line: RAW 264.7cells
Concentration: 0.625, 1.25, 2.5, 5, 10, 20, 40 μM
Incubation Time: 24 h
Result: The survival rate of RAW 264.7 cells was 97.44% at a concentration of 5 μM, indicating low cytotoxicity.

Western Blot Analysis[1]

Cell Line: RAW 264.7cells
Concentration: 2.5, 5, 10 μM
Incubation Time: 1 h
Result: Dose-dependent inhibited the expression of iNOS and COX-2 proteins.
Significantly inhibited LPS-induced phosphorylation of NF-κB p65 and degradation of IκBα, and reduced translocation of NF-κB p65 from the cytoplasm to the nucleus.
Significantly inhibited LPS-induced phosphorylation of ERK, JNK and p38 MAPK.

Real Time qPCR[1]

Cell Line: RAW 264.7cells
Concentration: 2.5, 5, 10 μM
Incubation Time: 24 h
Result: Significantly reduced mRNA expression levels of TNF-α, iNOS, IL-6 and COX-2.
分子量

535.72

Formula

C31H38FN3O2S
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

iNOS/COX-2-IN-1 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

iNOS/COX-2-IN-1COXNO SynthaseCyclooxygenaseNitric oxide synthasesNOSantiinflammatoryNF-κBMAPKRAW 264.7ArthritisInhibitorinhibitorinhibit

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产品名称：
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目录号：
HY-161513
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