2. Academic Validation
  3. Protective effect of 2-APB on testicular ischemia-reperfusion injury in rats

Protective effect of 2-APB on testicular ischemia-reperfusion injury in rats

  • J Urol. 2015 Mar;193(3):1036-41. doi: 10.1016/j.juro.2014.09.120.
Erhan Sari 1,  Hasan Aksit 2,  Haydar Ali Erken 3,  Arzu Yay 4,  Dilek Aksit 5,  Akin Soner Amasyali 6,  Onur Yildiz 2,  Ahmet Kucukyangoz 7,  Derya Akkus 4,  Kamil Seyrek 8
Affiliations

Affiliations

  • 1 Department of Urology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey. Electronic address: sarierhan@yahoo.com.
  • 2 Department of Biochemistry, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
  • 3 Department of Physiology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
  • 4 Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
  • 5 Department of Pharmacology, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
  • 6 Department of Urology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey.
  • 7 Department of Urology, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
  • 8 Department of Biochemistry, Faculty of Medicine, Veterinary Faculty, Balikesir University, Balikesir, Turkey.
PMID: 25444954 DOI: 10.1016/j.juro.2014.09.120
Abstract

Purpose: We performed biochemical and histopathological evaluations to assess the effects of 2-APB on ischemia-reperfusion induced testicular damage.

Materials and methods: A total of 28 rats were randomly divided into 4 groups, including sham treated, ischemia-reperfusion, ischemia-reperfusion plus 2 mg/kg 2-APB and ischemia-reperfusion plus 4 mg/kg 2-APB. Testicular tissue superoxide dismutase, glutathione, malondialdehyde, total antioxidant capacity and DNA fragmentation levels were determined. Testicular tissue samples were examined by histopathology and TUNEL staining.

Results: Mean superoxide dismutase, total antioxidant capacity and glutathione were significantly higher in the sham treated group than in the ischemia-perfusion group (p <0.05). Mean malondialdehyde and DNA fragmentation levels were significantly lower in the sham treated group than in the ischemia-reperfusion group (p <0.05). After 2-APB treatment superoxide dismutase, total antioxidant capacity and glutathione were significantly increased but malondialdehyde and DNA fragmentation levels were significantly decreased compared to the ischemia-reperfusion group (p <0.05). The number of TUNEL positive cells was significantly lower in the 2-APB treatment groups than in the ischemia-reperfusion group (p <0.05).

Conclusions: In rats 2-APB reduced the oxidative stress and Apoptosis caused by testicular ischemia-reperfusion injury. The testicular protective effect of 2-APB appears to be mediated through its antiapoptotic and antioxidative effects.

Keywords

2-aminoethoxydiphenyl borate; apoptosis; oxidative stress; reperfusion injury; testis.

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