2. Academic Validation
  3. Dehydroabietic Acid Suppresses Inflammatory Response Via Suppression of Src-, Syk-, and TAK1-Mediated Pathways

Dehydroabietic Acid Suppresses Inflammatory Response Via Suppression of Src-, Syk-, and TAK1-Mediated Pathways

  • Int J Mol Sci. 2019 Mar 29;20(7):1593. doi: 10.3390/ijms20071593.
Eunji Kim 1,  Young-Gyu Kang 2,  Yong-Jin Kim 3,  Tae Ryong Lee 4,  Byong Chul Yoo 5,  Minkyeong Jo 6,  Ji Hye Kim 7,  Jong-Hoon Kim 8,  Donghyun Kim 9,  Jae Youl Cho 10
Affiliations

Affiliations

  • 1 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. im144069@gmail.com.
  • 2 Basic Research & Innovation Division, R&D Center, AmorePacific Corporation, Yongin 17074, Korea. kangyg82@amorepacific.com.
  • 3 Basic Research & Innovation Division, R&D Center, AmorePacific Corporation, Yongin 17074, Korea. kjhmlkjhml@hanmail.net.
  • 4 Basic Research & Innovation Division, R&D Center, AmorePacific Corporation, Yongin 17074, Korea. trlee@amorepacific.com.
  • 5 Colorectal Cancer Branch, Research Institute, National Cancer Center, Goyang 10408, Korea. yoo_akh@ncc.re.kr.
  • 6 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. whalsrud1017@naver.com.
  • 7 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. kjhmlkjhml@hanmail.net.
  • 8 Department of Physiology, College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea. jhkim1@chonbuk.ac.kr.
  • 9 Basic Research & Innovation Division, R&D Center, AmorePacific Corporation, Yongin 17074, Korea. dhkim417@amorepacific.com.
  • 10 Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. jaecho@skku.edu.
PMID: 30934981 DOI: 10.3390/ijms20071593
Abstract

Dehydroabietic acid (DAA) is a naturally occurring diterpene resin acid derived from coniferous Plants such as Pinus and Picea. Various bioactive effects of DAA have been studied including Antibacterial, Antifungal, and Anticancer activities. However, the anti-inflammatory mechanism of DAA remains unclear. We evaluated the anti-inflammatory effect of DAA in macrophage cell lines. Dehydroabietic acid clearly reduced nitric oxide (NO) production and inflammatory gene expression decreased according to RT-PCR results. Dehydroabietic acid displayed anti-inflammatory activity at the transcriptional level in results from NF-κB- or AP-1-mediated luciferase assays. To identify the DAA target protein, we investigated NF-κB and AP-1 pathways by Western blotting analysis. Dehydroabietic acid suppressed the activity of proto-oncogene tyrosine protein kinase (Src) and spleen tyrosine kinase (Syk) in the NF-κB cascade and transforming growth factor beta-activated kinase 1 (TAK1) in the AP-1 cascade. Using overexpression strategies, we confirmed that DAA targeted these kinases. Our findings demonstrate the anti-inflammatory effects and molecular mechanism of DAA. This suggests that DAA has potential as a drug or supplement to ameliorate inflammation.

Keywords

AP-1; NF-κB; dehydroabietic acid (DAA); inflammation.

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