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  3. Dehydroabietic acid

Dehydroabietic acid  (Synonyms: 脱氢枞酸)

目录号: HY-N6869 纯度: 99.75%
COA 产品使用指南

Dehydroabietic acid 是一种可以从松树 (Pinus) 和云杉 (Picea) 中分离得到的二萜树脂酸。Dehydroabietic acid 具有抗菌、抗真菌、抗炎和抗癌活性。Dehydroabietic acid 是一种 PPAR-α/γ 双重激动剂和 PPAR-γ 部分激动剂,可以减轻小鼠因食用高脂肪饮食 (HFD) 而引起的胰岛素抵抗 (IR) 和肝脂肪变性。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Dehydroabietic acid Chemical Structure

Dehydroabietic acid Chemical Structure

CAS No. : 1740-19-8

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥770
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5 mg ¥700
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10 mg ¥1200
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20 mg ¥2000
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Other Forms of Dehydroabietic acid:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Dehydroabietic acid is a diterpene resin acid that can be isolated from Pinus and Picea. Dehydroabietic acid has anti-bacterial, anti-fungal, anti-inflammatory, and anticancer activities. Dehydroabietic acid is a dual PPAR-α/γ agonist and PPAR-γ partial agonist, which can attenuate insulin resistance (IR) and hepatic steatosis induced by HFD-consumption in mice[1][2].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
5637 IC50
27.59 μM
Compound: 1, DHAA
Cytotoxicity against human 5637 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human 5637 cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 23707051]
A549 IC50
> 10 μM
Compound: 19
Cytotoxicity in human A549 cells by SRB assay
Cytotoxicity in human A549 cells by SRB assay
[PMID: 26812172]
A549 IC50
> 50 μM
Compound: 1; DHA
Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
[PMID: 37122546]
A549 IC50
55.65 μM
Compound: DHAA
Cytotoxic activity against human A549 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
Cytotoxic activity against human A549 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
[PMID: 26706349]
A549 IC50
74.33 μM
Compound: 5
Cytotoxic activity against human A549 cells assessed as reduction in cell viability after 72 hrs by SRB assay
Cytotoxic activity against human A549 cells assessed as reduction in cell viability after 72 hrs by SRB assay
[PMID: 28011223]
A549 IC50
79.46 μM
Compound: DHA
Cytotoxicity against human A549 cells after 3 days by MTT assay
Cytotoxicity against human A549 cells after 3 days by MTT assay
[PMID: 24095745]
A549 IC50
79.46 μM
Compound: 4, DHA
Cytotoxicity against human A549 cells after 48 hrs by MTT assay
Cytotoxicity against human A549 cells after 48 hrs by MTT assay
[PMID: 23988357]
BEL-7404 tumor cell line IC50
28.72 μM
Compound: DHAA
Cytotoxic activity against human Bel7404 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
Cytotoxic activity against human Bel7404 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
[PMID: 26706349]
BEL-7404 tumor cell line IC50
34.7 μM
Compound: DHA
Cytotoxicity against human Bel7404 cells after 48 hrs by MTT assay
Cytotoxicity against human Bel7404 cells after 48 hrs by MTT assay
[PMID: 24565905]
EJ IC50
25.12 μM
Compound: 1, DHAA
Cytotoxicity against human EJ cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human EJ cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 23707051]
HCT-116 IC50
> 10 μM
Compound: 19
Cytotoxicity in human HCT116 cells by SRB assay
Cytotoxicity in human HCT116 cells by SRB assay
[PMID: 26812172]
HCT-116 IC50
30.25 μM
Compound: DHAA
Cytotoxic activity against human HCT116 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
Cytotoxic activity against human HCT116 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
[PMID: 26706349]
HCT-116 IC50
35.24 μM
Compound: DHA
Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay
Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay
[PMID: 24565905]
HeLa IC50
101 μg/mL
Compound: 4, dehydroabietic acid
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
[PMID: 19892441]
HeLa IC50
28.43 μM
Compound: 1, DHAA
Cytotoxicity against human HeLa cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 23707051]
HeLa IC50
29.35 μM
Compound: DHA
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
[PMID: 25462253]
HeLa IC50
29.35 μM
Compound: DHA
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
[PMID: 24565905]
HepG2 IC50
> 50 μM
Compound: 1; DHA
Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
[PMID: 37122546]
HepG2 IC50
> 50 μM
Compound: DHAA
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
[PMID: 28756264]
HepG2 IC50
76.76 μM
Compound: DHA
Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
[PMID: 24565905]
HepG2 IC50
85 μM
Compound: DHA
Cytotoxicity against human HepG2 cells after 3 days by MTT assay
Cytotoxicity against human HepG2 cells after 3 days by MTT assay
[PMID: 24095745]
HepG2 IC50
85 μM
Compound: 4, DHA
Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
[PMID: 23988357]
Jurkat IC50
25.51 μM
Compound: 1, DHAA
Cytotoxicity against human Jurkat cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human Jurkat cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 23707051]
Jurkat IC50
28 μg/mL
Compound: 4, dehydroabietic acid
Cytotoxicity against human Jurkat cells after 48 hrs by MTT assay
Cytotoxicity against human Jurkat cells after 48 hrs by MTT assay
[PMID: 19892441]
L02 IC50
> 50 μM
Compound: 1; DHA
Antiproliferative activity against human L02 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
Antiproliferative activity against human L02 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
[PMID: 37122546]
L02 IC50
> 50 μM
Compound: DHAA
Cytotoxicity against human HL-7702 cells after 48 hrs by MTT assay
Cytotoxicity against human HL-7702 cells after 48 hrs by MTT assay
[PMID: 28756264]
L6 IC50
101.3 μM
Compound: 1
Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric analysis
Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric analysis
10.1039/C5MD00498E
L6 IC50
151 μM
Compound: 1
Cytotoxicity against rat L6 cells after 72 hrs by Alamar blue assay
Cytotoxicity against rat L6 cells after 72 hrs by Alamar blue assay
10.1039/C5MD00498E
MCF7 IC50
> 50 μM
Compound: DHAA
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
[PMID: 28756264]
MGC-803 IC50
> 100 μM
Compound: DHA
Cytotoxicity against human MGC803 cells after 48 hrs by MTT assay
Cytotoxicity against human MGC803 cells after 48 hrs by MTT assay
[PMID: 25462253]
MGC-803 IC50
> 50 μM
Compound: 1; DHA
Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
[PMID: 37122546]
NCI-H460 IC50
> 50 μM
Compound: DHAA
Antiproliferative activity against human NCI-H460 cells after 48 hrs by MTT assay
Antiproliferative activity against human NCI-H460 cells after 48 hrs by MTT assay
[PMID: 28756264]
NCI-H460 IC50
75.05 μM
Compound: DHAA
Cytotoxic activity against human NCI-H460 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
Cytotoxic activity against human NCI-H460 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
[PMID: 26706349]
NCI-H460 IC50
80.53 μM
Compound: DHA
Cytotoxicity against human NCI-H460 cells after 48 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells after 48 hrs by MTT assay
[PMID: 24565905]
NCI-H460 IC50
84.53 μM
Compound: DHA
Cytotoxicity against human NCI-H460 cells after 3 days by MTT assay
Cytotoxicity against human NCI-H460 cells after 3 days by MTT assay
[PMID: 24095745]
NCI-H460 IC50
84.53 μM
Compound: DHA
Cytotoxicity against human NCI-H460 cells after 48 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells after 48 hrs by MTT assay
[PMID: 25462253]
NCI-H460 IC50
84.53 μM
Compound: 4, DHA
Cytotoxicity against human NCI-H460 cells after 48 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells after 48 hrs by MTT assay
[PMID: 23988357]
PC-3 IC50
28.1 μM
Compound: 1, DHAA
Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human PC3 cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 23707051]
PC-3 IC50
51.31 μM
Compound: 5
Cytotoxic activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by SRB assay
Cytotoxic activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by SRB assay
[PMID: 28011223]
SK-MEL-2 IC50
> 10 μM
Compound: 19
Cytotoxicity in human SK-MEL-2 cells by SRB assay
Cytotoxicity in human SK-MEL-2 cells by SRB assay
[PMID: 26812172]
SK-OV-3 IC50
> 10 μM
Compound: 19
Cytotoxicity in human SKOV3 cells by SRB assay
Cytotoxicity in human SKOV3 cells by SRB assay
[PMID: 26812172]
SK-OV-3 IC50
> 50 μM
Compound: DHAA
Antiproliferative activity against human SKOV3 cells after 48 hrs by MTT assay
Antiproliferative activity against human SKOV3 cells after 48 hrs by MTT assay
[PMID: 28756264]
SK-OV-3 IC50
65.06 μM
Compound: DHAA
Cytotoxic activity against human SKOV3 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
Cytotoxic activity against human SKOV3 cells assessed as inhibition of cell growth after 48 hrs by MTT assay
[PMID: 26706349]
SK-OV-3 IC50
65.96 μM
Compound: 5
Cytotoxic activity against human SKOV3 cells assessed as reduction in cell viability after 72 hrs by SRB assay
Cytotoxic activity against human SKOV3 cells assessed as reduction in cell viability after 72 hrs by SRB assay
[PMID: 28011223]
SK-OV-3 IC50
75 μM
Compound: DHA
Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay
Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay
[PMID: 24565905]
SK-OV-3 IC50
84 μM
Compound: DHA
Cytotoxicity against human SKOV3 cells after 3 days by MTT assay
Cytotoxicity against human SKOV3 cells after 3 days by MTT assay
[PMID: 24095745]
SK-OV-3 IC50
84 μM
Compound: 4, DHA
Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay
Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay
[PMID: 23988357]
T-24 IC50
> 50 μM
Compound: 1; DHA
Antiproliferative activity against human T24 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
Antiproliferative activity against human T24 cells assessed as reduction in cell viability measured for 24 hrs by MTT assay
[PMID: 37122546]
Vero IC50
20 μg/mL
Compound: 5
Concentration required for 50% inhibition of herpes simplex virus 2(HSV-2) using standard plaque reduction assay in vero cells
Concentration required for 50% inhibition of herpes simplex virus 2(HSV-2) using standard plaque reduction assay in vero cells
10.1016/S0960-894X(01)80236-5
Vero IC50
91 μg/mL
Compound: 4, dehydroabietic acid
Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay
Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay
[PMID: 19892441]
体外研究
(In Vitro)

Dehydroabietic acid (0-100 μM, 30 min) 可减少 RAW264.7 细胞中 NO 的产生[1]
Dehydroabietic acid (0-100 μM, 6 h) 可降低 RAW264.7 细胞中炎症介质的 mRNA 表达水平,包括诱导型一氧化氮 (iNOS) 和 TNF-α[1]
Dehydroabietic acid (0-100 μM, 24 h) 可降低 HEK293T 细胞中 MyD88 诱导的 NF-κBAP-1 转录活性[1]
Dehydroabietic acid (100 μM, 24 h) 可使 SrcSyk 过表达 HEK293T 细胞中的 SrcSyk 激酶失活[1]
Dehydroabietic acid (2.5-15 μM, 4 days) 以剂量依赖性方式促进 3T3-L1 脂肪细胞分化[2]
Dehydroabietic acid (10 μM, 0-6 days) 可增加 3T3-L1 细胞中 PPAR-γ 靶基因 (Glut-4 和 Cyp4a10) 的 mRNA 表达[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: AW264.7 cells
Concentration: 100 μM
Incubation Time: 5-60 min, 120-240 min
Result: Blocked the phosphorylation of IκBα at 5 and 15 min.
Reduced c-Jun N-terminal kinase (JNK) phosphorylation.
Reduced phosphorylated levels of mitogen-activated protein kinase kinase 4 (MKK4) and MKK7 at 60 mim.
Decreased the phosphorylation of MKK4 and MKK7 and their downstream protein JNK at 120 and 240 min.
体内研究
(In Vivo)

Dehydroabietic acid (10-20 mg/kg, 灌胃, 每日一次, 9 周) 在 HFD 小鼠中可减轻 HFD 诱导的肝脏脂肪变性和炎症[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: HFD mice[2]
Dosage: 10-20 mg/kg
Administration: i.g., daily, 9 weeks
Result: Decreased the levels of liver injury markers, ALT and AST.
Decreased blood TG, TC and LDL-c levels and increased the HDL-c levels.
Activated PPAR-α and its target gene which were reduced by HFD, including acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain and CPT1α.
Decreased mRNA expression of inflammatory factors commonly involved in liver diseases and injury (IL-1β, IL-6, TNF-α, COX-1 and COX-2).
Upregulated mRNA expression of PPAR-γ, Glut-4, Adipor, FSP27, ACOX-1, FABP4, Adiponectin.
分子量

300.44

Formula

C20H28O2

CAS 号
性状

固体

颜色

White to yellow

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 100 mg/mL (332.85 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3285 mL 16.6423 mL 33.2845 mL
5 mM 0.6657 mL 3.3285 mL 6.6569 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (8.32 mM); 悬浊液; 超声助溶

    此方案可获得 2.5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (8.32 mM); 悬浊液; 超声助溶

    此方案可获得 2.5 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

    2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.75%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3285 mL 16.6423 mL 33.2845 mL 83.2113 mL
5 mM 0.6657 mL 3.3285 mL 6.6569 mL 16.6423 mL
10 mM 0.3328 mL 1.6642 mL 3.3285 mL 8.3211 mL
15 mM 0.2219 mL 1.1095 mL 2.2190 mL 5.5474 mL
20 mM 0.1664 mL 0.8321 mL 1.6642 mL 4.1606 mL
25 mM 0.1331 mL 0.6657 mL 1.3314 mL 3.3285 mL
30 mM 0.1109 mL 0.5547 mL 1.1095 mL 2.7737 mL
40 mM 0.0832 mL 0.4161 mL 0.8321 mL 2.0803 mL
50 mM 0.0666 mL 0.3328 mL 0.6657 mL 1.6642 mL
60 mM 0.0555 mL 0.2774 mL 0.5547 mL 1.3869 mL
80 mM 0.0416 mL 0.2080 mL 0.4161 mL 1.0401 mL
100 mM 0.0333 mL 0.1664 mL 0.3328 mL 0.8321 mL
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产品名称:
Dehydroabietic acid
目录号:
HY-N6869
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