2. Academic Validation
  3. Chondroitin sulfate from sturgeon bone protects rat chondrocytes from hydrogen peroxide-induced damage by maintaining cellular homeostasis through enhanced autophagy

Chondroitin sulfate from sturgeon bone protects rat chondrocytes from hydrogen peroxide-induced damage by maintaining cellular homeostasis through enhanced autophagy

  • Int J Biol Macromol. 2020 Dec 1;164:2761-2768. doi: 10.1016/j.ijbiomac.2020.07.313.
Xi Zhang 1,  Qingsong Li 2,  Yingjiao Sun 3,  Lei Chen 4,  Jianfeng Wang 5,  Lishuang Liang 6
Affiliations

Affiliations

  • 1 Department of Pain, Qilu Hospital of Shandong University, Jinan 250101, China; Department of anesthesiology, The Second Hospital of Shandong University, Jinan 250101, China.
  • 2 Department of anesthesiology, The Second Hospital of Shandong University, Jinan 250101, China.
  • 3 Department of Pain, Qilu Hospital of Shandong University, Jinan 250101, China.
  • 4 Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan 250101, China.
  • 5 Department of Pain, Qilu Hospital of Shandong University, Jinan 250101, China. Electronic address: jianfengwang250101@163.com.
  • 6 Department of Pain, Qilu Hospital of Shandong University, Jinan 250101, China. Electronic address: lsliang@163.com.
PMID: 32763392 DOI: 10.1016/j.ijbiomac.2020.07.313
Abstract

We previously reported that treatment with chondroitin sulfate from sturgeon bone (CSSB) promoted anti-apoptotic activity in hydrogen peroxide (H2O2)-treated chondrocytes and had a protective effect on mitochondria. It is known that cells can repair damaged mitochondria through Autophagy, thus inhibiting the development of Apoptosis. Therefore, it is reasonable to speculate that CSSB treatment may inhibit chondrocyte Apoptosis via regulation of Autophagy. We observed the mitochondrial morphology of chondrocytes treated with different doses of CSSB, and confirmed that CSSB did not affect cell activity or cause damage to mitochondria. When compared with H2O2 treatment alone, CSSB treatment increased the clearance and repair of damaged mitochondria and promoted fusion of damaged mitochondria and lysosomes. CSSB treatment also increased the number of autolysosomes. However, these events could be blocked in chondrocytes pretreated with the Autophagy Inhibitor chloroquine, resulting in a decreased level of Autophagy and increased Apoptosis. These results suggest that CSSB treatment helps maintain intracellular homeostasis and prevent injury in chondrocytes treated with H2O2 by increasing Autophagy.

Keywords

Autophagy; Chondrocyte damage; Chondroitin sulfate; Hydrogen peroxide; Sturgeon bone.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z